Metabonomics study of atherosclerosis rats by ultra fast liquid chromatography coupled with ion trap-time of flight mass spectrometry

被引：112

作者：

Zhang, Fengxia ^{[2
,3
]}

Jia, Zhenhua ^{[1
]}

Gao, Peng ^{[3
]}

Kong, Hongwei ^{[3
]}

Li, Xiang ^{[3
]}

Chen, Jing ^{[3
]}

Yang, Qin ^{[3
]}

Yin, Peiyuan ^{[3
]}

Wang, Jiangshan ^{[3
]}

Lu, Xin ^{[3
]}

Li, Famei ^{[2
]}

Wu, Yiling ^{[1
]}

Xu, Guowang ^{[2
,3
]}

机构：

[1] Integrat Tradit & Western Med Res Acad Hebei Prov, Shijiazhuang 050035, Hebei Province, Peoples R China

[2] Shenyang Pharmaceut Univ, Sch Pharm, Dept Analyt Chem, Shenyang 110016, Peoples R China

[3] Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

来源：

TALANTA | 2009年 / 79卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Atherosclerosis; Metabonomics; UFLC/MS-IT-TOF; Plasma; Urine; Rats; POTENTIAL BIOMARKERS; UPLC-MS; LYSOPHOSPHATIDYLCHOLINE; METABOLOMICS; URINE; IDENTIFICATION; SPECTROSCOPY; DISCOVERY; SYSTEMS; LIVER;

D O I：

10.1016/j.talanta.2009.05.010

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An ultra fast liquid chromatography coupled with IT-TOF mass spectrometry (UFLC/MS-IT-TOF) metabonomic approach was employed to study the plasma and urine metabolic profiling of atherosclerosis rats. Acquired data were subjected to principal component analysis (PCA) for differentiating the atherosclerosis and the control groups. Potential biomarkers were screened by using S-plot and were identified by the accurate mass and MSn fragments information obtained from UFLC/MS-IT-TOF analysis. 12 metabolites in rat plasma and 8 metabolites in urine were identified as potential biomarkers. Concentrations of leucine, phenylalanine, tryptophan, acetylcarnitine, butyrylcarnitine, propionylcarnitine and spermine in plasma and 3-O-methyl-dopa, ethyl, N2-acetyl-L-argininate, leucylproline, glucuronate, t6A N(6)-(N-threonylcarbonyl)-adenosine and methyl-hippuric acid in urine decreased in atherosclerosis rats. Ursodeoxycholic acid, chenodeoxycholic acid, LPC (06:0), LPC (C18:0) and LPC (C18:1) in plasma and hippuric acid in urine were in higher levels in atherosclerosis rats. The alterated metabolites demonstrated abnormal metabolism of phenylalanine, tryptophan, bile acids and amino acids. This research proved that metabonomics is a promising tool for disease research. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：836 / 844

页数：9

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