Cytokine stimulation of human fetal hematopoietic cells

被引:5
作者
Ek, S
Westgren, M
Markling, L
Kjeldgaard, A
Ringden, O
机构
[1] Division of Fetal and Maternal Medicine, Department of Obstetrics and Gynecology, Huddinge
[2] Department of Clinical Immunology, Huddinge
[3] Department of Transplantation Surgery, Huddinge Hospital, Karolinska Institute, Huddinge
关键词
colony formation; cytokines; fetal transplantation; hematopoietic cells;
D O I
10.1159/000264333
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
The effects of interleukins 3 and 6, stem cell factor, and granulocyte-macrophage colony-stimulating factor on human fetal hematopoietic, bone marrow, and cord blood cells were studied on the basis of the colony-forming capacity. Fetal hematopoietic cells from 28 elective abortions, three bone marrow samples, and three cord blond samples were incubated with cytokines and investigated for the presence of BFU-E (burst-forming units - erythroid), CFU-GM (colony-forming units - granulocytes, macrophages), and CFU-GEMM (colony-forming units - granulocytes, erythrocytes, macrophages, megakaryocytes). Single and combined cytokines and preincubation versus adding cytokines in culture were investigated. Interleukin-6 alone had the most pronounced effect on BFU-E formation. All four cytokines in combination yielded the highest scores for CFU-GM (p < 0.05) and CFU-GEMM (p < 0.05), whereas BFU-E was not enhanced. The mode of cytokine exposure was not a determinant of colony formation.
引用
收藏
页码:318 / 325
页数:8
相关论文
共 17 条
[1]  
CROMBLEHOLME TM, 1994, SEMIN PERINATOL, V18, P376
[2]   TRANSPLANTATION OF FETAL CELLS [J].
CROMBLEHOLME, TM ;
LANGER, JC ;
HARRISON, MR ;
ZANJANI, ED .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1991, 164 (01) :218-230
[3]  
EGELAND T, 1993, TRANSPL P, V25, P1261
[4]  
EMERSON SG, 1989, BLOOD, V74, P49
[5]  
ENGLAND MA, 1990, NORMAL FETAL DEV COL
[6]  
GARDNER JD, 1990, BLOOD, V75, P2150
[7]   THE BIOLOGY OF INTERLEUKIN-6 [J].
KISHIMOTO, T .
BLOOD, 1989, 74 (01) :1-10
[8]  
METCALF D, 1987, EXP HEMATOL, V15, P288
[9]  
METCALF D, 1987, EXP HEMATOL, V15, P1
[10]   WHY DO HEMATOPOIETIC GROWTH-FACTOR RECEPTORS INTERACT WITH EACH OTHER [J].
NICOLA, NA .
IMMUNOLOGY TODAY, 1987, 8 (05) :134-140