Role of sphingosine kinase in Ca2+ signalling by epidermal growth factor receptor

被引：60

作者：

Heringdorf, DM ^{[1
]}

Lass, H ^{[1
]}

Kuchar, I ^{[1
]}

Alemany, R ^{[1
]}

Guo, YJ ^{[1
]}

Schmidt, M ^{[1
]}

Jakobs, KH ^{[1
]}

机构：

[1] Univ Essen Gesamthsch Klinikum, Inst Pharmakol, D-45122 Essen, Germany

来源：

FEBS LETTERS | 1999年 / 461卷 / 03期

关键词：

epidermal growth factor; sphingosine kinase; Ca2+ signalling; phospholipase C;

D O I：

10.1016/S0014-5793(99)01463-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Contribution of sphingosine kinase (SPK)-catalyzed production of sphingosine-1-phosphate (SPP), in comparison to phospholipase C (PLC), to Ca2+ signalling by epidermal growth factor (EGF) was studied in two HEK-293 cell clones (HEK2 and HEK3), expressing functional EGF receptors and exhibiting release of stored Ca2+ by intracellular SPP. In HEK3 cells, EGF increased [Ca2+](i) and stimulated both, SPK and PLC. [Ca2+](i) increase, but not PLC stimulation, was strongly reduced by SPK inhibition. In HEK2 cells, EGF similarly stimulated PLC, but did not increase [Ca2+](i) or stimulate SPK, suggesting that intracellular SPP production plays a major role for Ca2+ signalling by EGF in HEK-293 cells. Copyright (C) 1999 Federation of European Biochemical Societies.

引用

页码：217 / 222

页数：6

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