p97 ATPase, an ATPase involved in membrane fusion, interacts with DNA unwinding factor (DUF) that functions in DNA replication

被引：28

作者：

Yamada, T

Okuhara, K

Iwamatsu, A

Seo, H

Ohta, K

Shibata, T

Murofushi, H

机构：

[1] RIKEN, Cellular & Mol Biol Lab, Wako, Saitama 3510198, Japan

[2] Univ Tokyo, Fac Sci, Dept Biophys & Biochem, Bunkyo Ku, Tokyo 1130033, Japan

[3] Kirin Brewery Co Ltd, Cent Labs Key Technol, Sect Prot Chem, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan

来源：

FEBS LETTERS | 2000年 / 466卷 / 2-3期

基金：

日本科学技术振兴机构;

关键词：

DNA replication; DNA unwinding factor; p97; ATPase; Xenopus egg extract;

D O I：

10.1016/S0014-5793(99)01673-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA unwinding factor (DUF) unwinds duplex DNA and is supposed to function in DNA replication in Xenopus egg extracts, Here we report the isolation and analysis of a DUF-interacting factor. By immunoprecipitation, we found that p97 ATPase (p97) interacts with DUF in Xenopus egg extracts. This interaction was confirmed by the in vitro binding of purified p97 with DUF, When sperm chromatin was added to Xenopus egg extracts to construct nuclei active in DNA replication, p97 was incorporated into the nuclei. These data suggest that the complex of DUF and p97 may function in DNA replication. (C) 2000 Federation of European Biochemical Societies.

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页码：287 / 291

页数：5

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