A second form of infectious Bursal disease virus-associated tubule contains VP4

被引：37

作者：

Granzow, H ^{[1
]}

Birghan, C ^{[1
]}

Mettenleiter, TC ^{[1
]}

Beyer, J ^{[1
]}

Kollner, B ^{[1
]}

Mundt, E ^{[1
]}

机构：

[1] FED RES CTR VIRUS DIS ANIM, FRIEDRICH LOEFFLER INST, INST MOL & CELLULAR VIROL, D-17498 INSEL RIEMS, GERMANY

来源：

JOURNAL OF VIROLOGY | 1997年 / 71卷 / 11期

关键词：

D O I：

10.1128/JVI.71.11.8879-8885.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Preparations of density gradient-purified infectious bursal disease virus (IBDV) were found to contain full and empty icosahedral virions, type I tubules with a diameter of about 60 nm, and type II tubules 24 to 26 nm in diameter. By immunoelectron microscopy we demonstrate that virions and both types of tubular structures specifically react with anti-IBDV serum. In infected cells intracytoplasmic and intranuclear type II tubules reacted exclusively with an anti-VP4 monoclonal antibody, as did type II tubules in virion preparations. The immunofluorescence pattern with the anti-VP4 antibody correlated with electron microscopical findings. Neither purified extracellular nor intracellular virions were labeled with the anti-VP4 MAb. Our data show that the type II tubules contain VP4 and suggest that VP4 is not part of the virus particle.

引用

页码：8879 / 8885

页数：7

共 42 条

[1] DELETION MAPPING AND EXPRESSION IN ESCHERICHIA-COLI OF THE LARGE GENOMIC SEGMENT OF A BIRNAVIRUS [J].