Glycosylphosphatidylinositol anchors represent the major carbohydrate modification in proteins of intraerythrocytic stage Plasmodium falciparum

被引:112
作者
Gowda, DC
Gupta, P
Davidson, EA
机构
[1] Dept. of Biochem. and Molec. Biology, Georgetown University Medical Center, Washington
[2] Dept. of Biochem. and Molec. Biology, Georgetown University Medical Center, Washington, DC 20007, 3900 Reservoir Rd., NW
关键词
D O I
10.1074/jbc.272.10.6428
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The nature and extent of carbohydrate modification in intraerythrocytic stage Plasmodium falciparum proteins have been controversial. This study describes the characterization of the carbohydrates in intraerythrocytic P. falciparum proteins and provides an overall picture of the nature of carbohydrate modification in the parasite proteins. P. falciparum strains were metabolically labeled with radioactive sugar precursors and ethanolamine at different developmental stages. The individual parasite proteins separated on SDS-polyacrylamide gels and whole parasite cell lysates were analyzed for the carbohydrate moieties. The results established the following: 1) glycosylphosphatidylinositol (GPI) anchors represent the major carbohydrate modification in the intraerythrocytic stage P. falciparum proteins; 2) in contrast to previous reports, O-linked carbohydrates are either absent or present only at very low levels in the parasite; and 3) P. falciparum contains low levels of N-glycosylation capability. The amount of N-linked carbohydrates in whole parasite proteins is approximately 6% compared with the GPI anchors attached to proteins based on radioactive GlcN incorporated into the proteins. The glycan cores of multiple parasite protein GPI anchors are all similar, consisting of protein-ethanolamine-phosphate-(Man-alpha-1-2)6Man-alpha-1-2Man-alpha-1-6Man-alpha-1-4GlcN. The fourth Man residues distal to GlcN of the GPI anchor glycan cores contain unidentified substituents that are susceptible to conditions of nitrous acid deamination. This unusual structural feature may contribute to the reported pathogenic properties of the P. falciparum GPI anchors.
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页码:6428 / 6439
页数:12
相关论文
共 66 条
  • [1] INDUCTION OF THE PROTEOLYTIC ACTIVITY OF A MEMBRANE-PROTEIN IN PLASMODIUM-FALCIPARUM BY PHOSPHATIDYL INOSITOL-SPECIFIC PHOSPHOLIPASE-C
    BRAUNBRETON, C
    ROSENBERRY, TL
    DASILVA, LP
    [J]. NATURE, 1988, 332 (6163) : 457 - 459
  • [2] GLYCOLIPID ANCHORAGE OF PLASMODIUM-FALCIPARUM SURFACE-ANTIGENS
    BRETON, CB
    ROSENBERRY, TL
    DASILVA, LHP
    [J]. RESEARCH IN IMMUNOLOGY, 1990, 141 (08): : 743 - 755
  • [3] CARLSON DM, 1966, J BIOL CHEM, V241, P2984
  • [4] FLUOROGRAPHIC DETECTION OF RADIOACTIVITY IN POLYACRYLAMIDE GELS WITH THE WATER-SOLUBLE FLUOR, SODIUM-SALICYLATE
    CHAMBERLAIN, JP
    [J]. ANALYTICAL BIOCHEMISTRY, 1979, 98 (01) : 132 - 135
  • [5] A recombinant Baculovirus 42-kilodalton c-terminal fragment of Plasmodium falciparum merozoite surface protein 1 protects Aotus monkeys against malaria
    Chang, SP
    Case, SE
    Gosnell, WL
    Kramer, KJ
    Tam, LQ
    Hashiro, CQ
    Nikaido, CM
    Gibson, HL
    LeeNg, CT
    Barr, PJ
    Yokota, BT
    Hui, GSN
    [J]. INFECTION AND IMMUNITY, 1996, 64 (01) : 253 - 261
  • [6] IMMUNIZATION WITH SYNTHETIC PEPTIDES OF A PLASMODIUM-FALCIPARUM SURFACE-ANTIGEN INDUCES ANTIMEROZOITE ANTIBODIES
    CHEUNG, A
    LEBAN, J
    SHAW, AR
    MERKLI, B
    STOCKER, J
    CHIZZOLINI, C
    SANDER, C
    PERRIN, LH
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (21) : 8328 - 8332
  • [7] CHOU CF, 1992, J BIOL CHEM, V267, P3901
  • [8] MAPPING OF THE REGION PREDOMINANTLY RECOGNIZED BY ANTIBODIES TO THE PLASMODIUM-FALCIPARUM MEROZOITE SURFACE-ANTIGEN MSA-1
    COOPER, JA
    COOPER, LT
    SAUL, AJ
    [J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1992, 51 (02) : 301 - 312
  • [9] PRESENCE OF O-GLYCOSYLATED GLYCOPROTEINS IN THE PLASMODIUM-FALCIPARUM PARASITE
    DAYALDRAGER, R
    HOESSLI, DC
    DECRIND, C
    DELGUIDICE, G
    LAMBERT, PH
    NASIRUDDIN
    [J]. CARBOHYDRATE RESEARCH, 1991, 209 : C5 - C8
  • [10] APPARENT LACK OF N-GLYCOSYLATION IN THE ASEXUAL INTRAERYTHROCYTIC STAGE OF PLASMODIUM-FALCIPARUM
    DIECKMANNSCHUPPERT, A
    BENDER, S
    ODENTHALSCHNITTLER, M
    BAUSE, E
    SCHWARZ, RT
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 205 (02): : 815 - 825