Formation of supported membranes from vesicles

被引:405
作者
Keller, CA [1 ]
Glasmästar, K
Zhdanov, VP
Kasemo, B
机构
[1] Chalmers Univ Technol, Dept Appl Phys, S-41296 Gothenburg, Sweden
[2] Univ Gothenburg, S-41296 Gothenburg, Sweden
[3] Russian Acad Sci, Boreskov Inst Catalysis, Novosibirsk 630090, Russia
关键词
D O I
10.1103/PhysRevLett.84.5443
中图分类号
O4 [物理学];
学科分类号
0702 ;
摘要
Using a combination of the quartz crystal microbalance and surface plasmon resonance techniques, we have studied the spontaneous formation of supported lipid bilayers from small (similar to 25 nm) unilamellar vesicles. Together these experimental methods measure the amount of lipid adsorbed on the surface and the amount of water trapped by the lipid. With this approach, we have, for the first time, been able to observe in detail the progression from the adsorption of intact vesicles to rupture and bilayer formation. Monte Carlo simulations reproduce the data.
引用
收藏
页码:5443 / 5446
页数:4
相关论文
共 27 条
[1]   SIMPLE METHOD FOR PREPARATION OF HOMOGENEOUS PHOSPHOLIPID VESICLES [J].
BARENHOLZ, Y ;
GIBBES, D ;
LITMAN, BJ ;
GOLL, J ;
THOMPSON, TE ;
CARLSON, FD .
BIOCHEMISTRY, 1977, 16 (12) :2806-2810
[2]   SELF-ASSEMBLY OF COVALENTLY ANCHORED PHOSPHOLIPID SUPPORTED MEMBRANES BY USE OF DODA-SUC-NHS-LIPIDS [J].
BRINK, G ;
SCHMITT, L ;
TAMPE, R ;
SACKMANN, E .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1994, 1196 (02) :227-230
[3]   Cytochrome c oxidase immobilized in stable supported lipid bilayer membranes [J].
Burgess, JD ;
Rhoten, MC ;
Hawkridge, FM .
LANGMUIR, 1998, 14 (09) :2467-2475
[4]   Surface plasmon resonance analysis at a supported lipid monolayer [J].
Cooper, MA ;
Try, AC ;
Carroll, J ;
Ellar, DJ ;
Williams, DH .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1998, 1373 (01) :101-111
[5]   Formation and spreading of lipid bilayers on planar glass supports [J].
Cremer, PS ;
Boxer, SG .
JOURNAL OF PHYSICAL CHEMISTRY B, 1999, 103 (13) :2554-2559
[6]  
Czanderna A.W., 1984, APPL PIEZOELECTRIC Q, V7, P1
[7]   SPREADING OF GIANT VESICLES ON MODERATELY ADHESIVE SUBSTRATES BY FINGERING - A REFLECTION INTERFERENCE CONTRAST MICROSCOPY STUDY [J].
FEDER, TJ ;
WEISSMULLER, G ;
ZEKS, B ;
SACKMANN, E .
PHYSICAL REVIEW E, 1995, 51 (04) :3427-3433
[8]   COVALENT ATTACHMENT OF FUNCTIONALIZED LIPID BILAYERS TO PLANAR WAVE-GUIDES FOR MEASURING PROTEIN-BINDING TO BIOMIMETIC MEMBRANES [J].
HEYSEL, S ;
VOGEL, H ;
SANGER, M ;
SIGRIST, H .
PROTEIN SCIENCE, 1995, 4 (12) :2532-2544
[9]   STUDIES ON PHOSPHATIDYLCHOLINE VESICLES . FORMATION AND PHYSICAL CHARACTERISTICS [J].
HUANG, CH .
BIOCHEMISTRY, 1969, 8 (01) :344-+
[10]   Self assembly driven by hydrophobic interactions at alkanethiol monolayers: mechanism of formation of hybrid bilayer membranes [J].
Hubbard, JB ;
Silin, V ;
Plant, AL .
BIOPHYSICAL CHEMISTRY, 1998, 75 (03) :163-176