Isolation and characterization of the epothilone biosynthetic gene cluster from Sorangium cellulosum

被引：131

作者：

Julien, B ^{[1
]}

Shah, S ^{[1
]}

Ziermann, R ^{[1
]}

Goldman, R ^{[1
]}

Katz, L ^{[1
]}

Khosla, C ^{[1
]}

机构：

[1] Kosan Biosci Inc, Hayward, CA 94545 USA

来源：

GENE | 2000年 / 249卷 / 1-2期

关键词：

myxobacteria; non-ribosomal peptide synthetase; polyketide; polyketide synthase; P450; enzyme;

D O I：

10.1016/S0378-1119(00)00149-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The epothilone biosynthetic gene cluster was isolated from Sorangium cellulosum strain SMP44. The gene cluster contains seven genes and spans approx. 56 kb. The genes encoding the PKS, epoA, epoC, epoD, epoE, and epoF, are divided into nine modules. The EpoB protein is a non-ribosomal peptide synthetase (NRPS) that catalyzes formation of the thiazole found in the epothilones. EpoK is a P450 enzyme responsible for the epoxidation of epothilones C and D to epothilones A and B, respectively. EpoK was expressed in Escherichia coli, and the purified protein was shown to convert epothilone D to epothilone B in vitro. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：153 / 160

页数：8

共 29 条

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