Intratracheal instillation of bone marrow-derived cell in an experimental model of silicosis

被引：41

作者：

Lassance, Roberta M.

Prota, Luiz Felipe M.

Maron-Gutierrez, Tatiana

Garcia, Cristiane S. N. B. ^{[2
]}

Abreu, Soraia C. ^{[2
]}

Passaro, Caroline P. ^{[2
]}

Xisto, Debora G. ^{[2
]}

Castiglione, Raquel C.

Carreira Junior, Humberto ^{[2
,3
]}

Ornellas, Debora Santos ^{[2
]}

Santana, Maria Cristina E. ^{[2
]}

Souza, Sergio A. L. ^{[3
]}

Gutfilen, Bianca ^{[3
]}

Fonseca, Lea Miriam B. ^{[3
]}

Rocco, Patricia R. M. ^{[2
]}

Morales, Marcelo M. ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho CCS, Lab Fisiol Celular & Mol, Ilha Fundao, BR-21941902 Rio De Janeiro, Brazil

[2] Inst Biofis Carlos Chagas Filho, Lab Pulm Invest, Rio De Janeiro, Brazil

[3] Univ Fed Rio de Janeiro, Hosp Univ Clementino Fraga Filho, Ctr Ciencias Saude, Ilha Fundao, BR-21941902 Rio De Janeiro, Brazil

来源：

RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY | 2009年 / 169卷 / 03期

关键词：

Pulmonary mechanics; Morphometry; Silicosis; Bone marrow-derived cells; MESENCHYMAL STEM-CELLS; THERAPY POSITION STATEMENT; STROMAL CELLS; PULMONARY-FIBROSIS; INTERNATIONAL-SOCIETY; LUNG INJURY; RAT MODEL; ENGRAFTMENT; MECHANISMS; EXPOSURE;

D O I：

10.1016/j.resp.2009.09.004

中图分类号：

Q4 [生理学];

学科分类号：

071003 [生理学];

摘要：

The time course of lung mechanics, histology, and inflammatory and fibrogenic mediators are analysed after intratracheal instillation (IT) of bone marrow-derived cells (BMDC) in a model of silicosis. C57BL/6 mice were randomly divided into SIL (silica, 20 mg IT) and control (CTRL) groups (saline IT). At day 15, mice received saline or BMDC (2 x 10(6) cells) IT. The biodistribution of technetium-99m BMDC was higher in lungs compared with other organs. At days 30 and 60, lung mechanics, the area of granulomatous nodules, and mRNA expression of IL-1 beta and TGF-beta were higher in SIL than CTRL animals. BMDC minimized changes in lung mechanics, the area of granulomatous nodules, and total cell infiltration at day 30, but these effects were no longer observed at day 60. Conversely, BMDC avoided the expression of IL-1 beta at days 30 and 60 and TGF-beta only at day 30. In conclusion, BMDC therapy improved lung mechanics and histology, but this beneficial effect was not maintained in the course of injury. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：227 / 233

页数：7

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