Rapid, multifluorescent TCRG Vγ and Jγ typing:: application to T cell acute lymphoblastic leukemia and to the detection of minor clonal populations

被引:45
作者
Delabesse, E
Burtin, ML
Millien, C
Madonik, A
Arnulf, B
Beldjord, K
Valensi, F
Macintyre, EA
机构
[1] Hop Necker Enfants Malad, CNRS UMR8603, Paris, France
[2] Univ Paris 05, Paris, France
[3] PE Biosyst, Foster City, CA USA
关键词
T-ALL; TCR gamma; PCR diagnosis; fluorescent PCR;
D O I
10.1038/sj.leu.2401750
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Detection of clonal T cell receptor gamma (TCRG) gene rearrangements by PCR is widely used in both the diagnostic assessment of lymphoproliferative disorders and the follow-up of acute lymphoblastic leukaemia (ALL), when residual positivity in excess of 10(-3) at morphological complete remission is increasingly recognised to be an independent marker of poor prognosis. This is largely based on specific detection of V-J rearrangements from childhood cases. We describe rapid, multifluorescent V gamma and J gamma PCR typing of multiplex amplified diagnostic samples, as applied to 46 T-ALL. These strategies allow selected analysis of appropriate cases, immediate identification of V gamma and J gamma segments in over 95% of alleles, improved resolution and precision sizing and a sensitivity of detection at the 10(-2)-10(-3) level. We demonstrate preferential V-J combinations but no difference in V-J usage between children and adults, nor between SIL-TALI-negative and -positive cases. A combination of fluorescent multiplex and V gamma-J gamma-specific monoplex follow-up, as described here, will allow detection of both significant clonal evolution and of the diagnostic clone at a level of prognostic significance, by techniques which can readily be applied to large-scale prospective studies for which real-time analysis is required.
引用
收藏
页码:1143 / 1152
页数:10
相关论文
共 25 条
[1]   ASSESSMENT OF CLONAL EVOLUTION AT IG/TCR LOCI IN ACUTE LYMPHOBLASTIC-LEUKEMIA BY SINGLE-STRAND CONFORMATION POLYMORPHISM STUDIES AND HIGHLY RESOLUTIVE PCR DERIVED METHODS - IMPLICATION FOR A GENERAL STRATEGY OF MINIMAL RESIDUAL DISEASE DETECTION [J].
BARUCHEL, A ;
CAYUELA, JM ;
MACINTYRE, E ;
BERGER, R ;
SIGAUX, F .
BRITISH JOURNAL OF HAEMATOLOGY, 1995, 90 (01) :85-93
[2]  
Castellanos A, 1998, LEUKEMIA, V12, P251
[3]   Clinical significance of minimal residual disease in childhood acute lymphoblastic leukemia [J].
Cavé, H ;
ten Bosch, JV ;
Suciu, S ;
Guidal, C ;
Waterkeyn, C ;
Otten, J ;
Bakkus, M ;
Thielemans, K ;
Grandchamp, B ;
Vilmer, E .
NEW ENGLAND JOURNAL OF MEDICINE, 1998, 339 (09) :591-598
[4]  
CHEN Z, 1988, BLOOD, V72, P776
[5]  
DAURIOL L, 1989, LEUKEMIA, V3, P155
[6]   Simultaneous SIL-TAL1 RT-PCR detection of all tald deletions and identification of novel tald variants [J].
Delabesse, E ;
Bernard, M ;
Landman-Parker, J ;
Davi, F ;
Leboeuf, D ;
Varet, B ;
Valensi, F ;
Macintyre, EA .
BRITISH JOURNAL OF HAEMATOLOGY, 1997, 99 (04) :901-907
[7]   RESTRICTED DIVERSITY OF V-GAMMA-9-JP REARRANGEMENTS IN UNSTIMULATED HUMAN GAMMA/DELTA T LYMPHOCYTE-T [J].
DELFAU, MH ;
HANCE, AJ ;
LECOSSIER, D ;
VILMER, E ;
GRANDCHAMP, B .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1992, 22 (09) :2437-2443
[8]   Residual disease detection using fluorescent polymerase chain reaction at 20 weeks of therapy predicts clinical outcome in childhood acute lymphoblastic leukemia [J].
Evans, PAS ;
Short, MA ;
Owen, RG ;
Jack, AS ;
Forsyth, PD ;
Shiach, CR ;
Kinsey, S ;
Morgan, GJ .
JOURNAL OF CLINICAL ONCOLOGY, 1998, 16 (11) :3616-3627
[9]   Multiplex PCR for rapid detection of T-cell receptor-gamma chain gene rearrangements in patients with lymphoproliferative diseases [J].
Fodinger, M ;
Buchmayer, H ;
Schwarzinger, I ;
Simonitsch, I ;
Winkler, K ;
Jager, U ;
Knobler, R ;
Mannhalter, C .
BRITISH JOURNAL OF HAEMATOLOGY, 1996, 94 (01) :136-139
[10]   THE V-GAMMA LOCUS OF THE HUMAN T-CELL RECEPTOR GAMMA-GENE REPERTOIRE POLYMORPHISM OF THE 1ST VARIABLE GENE SEGMENT SUBGROUP [J].
FONT, MP ;
CHEN, Z ;
BORIES, JC ;
DUPARC, N ;
LOISEAU, P ;
DEGOS, L ;
CANN, H ;
COHEN, D ;
DAUSSET, J ;
SIGAUX, F .
JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 168 (04) :1383-1394