Crystallization and preliminary X-ray analysis of human brain hexokinase

被引：5

作者：

Aleshin, AE ^{[1
]}

Zeng, CB ^{[1
]}

Fromm, HJ ^{[1
]}

Honzatko, RB ^{[1
]}

机构：

[1] IOWA STATE UNIV,DEPT BIOCHEM & BIOPHYS,AMES,IA 50011

来源：

FEBS LETTERS | 1996年 / 391卷 / 1-2期

关键词：

brain hexokinase; hexokinase type I; X-ray crystallography; crystallization;

D O I：

10.1016/0014-5793(96)00688-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human brain hexokinase type I, expressed in Escherichia coli, has been crystallized from polyethylene glycol 8000 in the presence of inorganic phosphate. The crystals are hexagonal needles of diameter 0.25 mm, diffracting to a resolution of 3.5 Angstrom on a rotating-anode/area-detector system. The crystals belong to the space group P3(1)21/P3(2)21 with cell dimensions a = b = 171.5 Angstrom and c = 99.4 Angstrom. The specific volume of the crystal is 4.2 Angstrom(3)/Da, suggesting an asymmetric unit with a single 100 kDa molecule and a solvent content of 71% by volume or two molecules of hexokinase with a solvent content of 41%. The complex of hexokinase with glucose crystallizes under similar conditions, giving crystals of the same morphology.

引用

页码：9 / 10

页数：2

共 24 条

[1] REFINED MODEL OF SUGAR BINDING-SITE OF YEAST HEXOKINASE-B [J].