Equal performance of TaqMan, MGB, molecular beacon, and SYBR green-based detection assays in detection and quantification of roundup ready soybean

被引：43

作者：

Andersen, Charlotte Boydler

Holst-Jensen, Arne

Berdal, Knut Gunnar

Thorstensen, Tage

Tengs, Torstein

机构：

[1] Natl Vet Inst, Sect Food & Feed Microbiol, N-0454 Oslo, Norway

[2] Univ Oslo, Dept Mol Biosci, N-0316 Oslo, Norway

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2006年 / 54卷 / 26期

关键词：

event specific detection; genetically modified organisms; GMO; method performance; real-time PCR; reliability; RoundupReady soybean;

D O I：

10.1021/jf061987c

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

We have tested and compared the performance of 12 different assays representing four different real-time polymerase chain reaction (PCR) chemistries in the context of genetically modified organism detection. Several different molecular beacon, SYBR Green, TaqMan, and MGB assays were designed for the event specific detection and quantification of the 3' integration junction of GTS 40-3-2 (Roundup Ready) soybean. Sensitivity as well as robustness in the presence of background DNA were tested. None of the PCR-based approaches appeared to be significantly better than any of the other, but the molecular beacon assays had the lowest efficiency and also seemed more sensitive to changes in experimental setup.

引用

页码：9658 / 9663

页数：6

共 29 条

[1]

Arumuganathan K, 1991, PLANT MOL BIOL REP, V9, P208, DOI [DOI 10.1007/BF02672069, 10.1007/BF02672069]

[2] Roundup Ready® soybean event-specific real-time quantitative PCR assay and estimation of the practical detection and quantification limits in GMO analyses [J].