A fish antimicrobial peptide, tilapia hepcidin TH2-3, shows potent antitumor activity against human fibrosarcoma cells

被引：162

作者：

Chen, Jyh-Yih ^{[1
,2
]}

Lin, Wei-Ju ^{[1
]}

Lin, Tai-Lang ^{[3
]}

机构：

[1] Acad Sinica, Inst Cellular & Organism Biol, Marine Res Stn, Jiaushi 262, Ilan, Taiwan

[2] Natl Taiwan Ocean Univ, Inst Biosci & Biotechnol, Chilung 202, Taiwan

[3] Acad Sinica, Inst Cellular & Organism Biol, Taipei 110, Taiwan

来源：

PEPTIDES | 2009年 / 30卷 / 09期

关键词：

Hepcidin; Tumor cell inhibition; Lytic peptide; IRON-METABOLISM; KEY REGULATOR; EXPRESSION;

D O I：

10.1016/j.peptides.2009.06.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

As part of a continuing search for potential anticancer drug candidates from antimicrobial peptides of marine organisms, tilapia (Oreochromis mossambicus) hepcidin TH2-3 was evaluated in several tumor cell lines. The results indicated that TH2-3, a synthetic 20-mer antimicrobial peptide, specifically inhibited human fibrosarcoma cell (HT1080 cell line) proliferation and migration. The way in which TH23 inhibited HT1080 cell growth was then studied. TH2-3 inhibited HT1080 cell growth in a concentration-dependent manner according to an MTT analysis. which was confirmed by a soft-agar assay and AO/EtBr staining. Scanning electron microscopy revealed that TH2-3 caused lethal membrane disruption in HT1080 cancer cells, and a wound healing assay supported that TH2-3 decreased the migration of HT1080 cells. In addition, c-Jun mRNA expression was downregulated after treatment with TH2-3 for 48-96 h compared to the untreated group. These findings suggest a mechanism of cytotoxic action of TH2-3 and indicate that TH2-3 may be a promising chemotherapeutic agent against human fibrosarcoma cells. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：1636 / 1642

页数：7

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