Type III secretion-dependent modulation of innate immunity as one of multiple factors regulated by Pseudomonas aeruginosa RetS

被引:32
作者
Zolfaghar, Irandokht
Evans, David J.
Ronaghi, Reza
Fleiszig, Suzanne M. J. [1 ]
机构
[1] Univ Calif Berkeley, Sch Optometry, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Grad Grp Microbiol Infect Dis & Immun & Vis Sci, Berkeley, CA 94720 USA
[3] Touro Univ, Coll Pharm, Vallejo, CA 94592 USA
关键词
CORNEAL EPITHELIAL-CELLS; ACUTE LUNG INJURY; IN-VITRO; POLYMORPHONUCLEAR NEUTROPHILS; EXOT CONTRIBUTES; SYSTEMIC SPREAD; ACUTE PNEUMONIA; EXOENZYME-S; INFECTION; VIRULENCE;
D O I
10.1128/IAI.01891-05
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Mutation of retS (rtsM) of Pseudomonas aeruginosa strain PA103 reduces its virulence in both ocular and respiratory murine models of infection. In vitro, retS mutants exhibit loss of the ExsA-regulated type III secretion system (TTSS), reduced twitching motility, and a decrease in association with, invasion of, and survival within corneal epithelial cells. In addition, transcription of multiple other virulence genes is positively and negatively affected by retS mutation. Since our published data show that ExoU and ExoT, the two TTSS effectors encoded by strain PA103, each confer virulence in this corneal model, we hypothesized that loss of virulence of retS mutants follows loss of type III secretion. Corneal pathology, bacterial colonization, and phagocyte infiltration were compared for wild-type PA103, retS mutants, and various TTSS mutants after infection with similar to 10(6) CFU bacteria. Results showed that either a retS or an exsA (TTSS) mutation delayed disease progression, as illustrated by reduced severity scores and colonization levels during the first 48 h postinfection. Surprisingly, retS mutant infections then became more severe than those involving exsA mutants. By day 7, colonization levels of retS mutants even surpassed those of wild-type bacteria (more than twofold, P = 0.028). Although retS mutants caused more severe opacification of central corneas than both the wild type and the exsA mutants, neither mutant caused the peripheral ring opacity commonly associated with wild-type infection, suggesting that the TTSS was involved. Histological experiments with retS and various TTSS mutants showed that ring opacification required ExoU but not ExoT and that it consisted of dense polymorphonuclear phagocyte infiltration at the corneal periphery and the absence of any cell type in the central cornea. These data suggest that these P. aeruginosa TTSS effectors have different effects on innate immunity and that RetS influences virulence beyond its effects on the TTSS.
引用
收藏
页码:3880 / 3889
页数:10
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