Serum of patients with septic shock stimulates the expression of Trem-1 on U937 monocytes

被引：31

作者：

Dimopoulou, I. ^{[2
]}

Orfanos, S. E. ^{[2
]}

Pelekanou, A. ^{[1
]}

Kotanidou, A. ^{[3
]}

Livaditi, O. ^{[3
]}

Augustatou, C. ^{[2
]}

Zervou, M. ^{[2
]}

Douka, E. ^{[2
]}

Theodorakopoulou, M. ^{[2
]}

Karagianni, V. ^{[1
]}

Douzinas, E. ^{[4
]}

Armaganidis, A. ^{[2
]}

Giamarellos-Bourboulis, E. J. ^{[1
]}

机构：

[1] Univ Athens, Sch Med, Dept Internal Med 4, GR-10679 Athens, Greece

[2] Univ Athens, Sch Med, Dept Crit Care Med 2, GR-10679 Athens, Greece

[3] Univ Athens, Sch Med, Dept Crit Care Med 1, GR-10679 Athens, Greece

[4] Univ Athens, Sch Med, Dept Crit Care Med 3, GR-10679 Athens, Greece

来源：

INFLAMMATION RESEARCH | 2009年 / 58卷 / 03期

关键词：

Monocytes; Infection; Shock; Cytokines; Inflammation; VENTILATOR-ASSOCIATED PNEUMONIA; MYELOID CELLS-1; INFLAMMATORY RESPONSES; SEPSIS; MEDIATOR; CULTURES;

D O I：

10.1007/s00011-008-7039-4

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

To describe the concentrations of sTREM-1 in patients with sepsis and to explore the effects of their serum on the expression of TREM-1 on U937 monocytes. Blood was sampled at regular time intervals in 56 patients with sepsis. Concentrations of tumour necrosis factor-alpha (TNF alpha), interleukin-1beta (IL-1 alpha), IL-6, IL-8, IL-10 and IL-12p70 and sTREM-1 were measured. U937 monocytes were incubated in the presence of serum at sepsis onset. Median sTREM-1 concentration on day 1 for patients with septic shock was 915 pg/ml and 228.5 pg/ml for those without shock (p = 0.002). TNF alpha, IL-1 alpha, IL-6, IL-8 and IL-10 did not differ between them. A positive correlation was found between changes of sTREM-1 and SOFA scores from day 1 to 7. Sera of patients with septic shock evoked a significant increase of the expression of TREM-1. The concentrations of TNF alpha and IL-8 in supernatants increased only after stimulating with sera of patients without shock, but not after stimulating with sera of patients with shock. Levels of sTREM-1 correlated with sepsis severity. sTREM-1 is considerably higher in patients with shock compared to patients without shock. The serum of shocked patients could stimulate the expression of TREM-1 on U937 monocytes.

引用

页码：127 / 132

页数：6

共 16 条

[1] A role for triggering receptor expressed on myeloid cells-1 in host defense during the early-induced and adaptive phases of the immune response [J].

Bleharski, JR ;

Kiessler, V ;

Buonsanti, C ;

Sieling, PA ;

Stenger, S ;

Colonna, M ;

Modlin, RL .

JOURNAL OF IMMUNOLOGY, 2003, 170 (07) :3812-3818

[2] Cutting edge: Inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes [J].

Bouchon, A ;

Dietrich, J ;

Colonna, M .

JOURNAL OF IMMUNOLOGY, 2000, 164 (10) :4991-4995

[3] TREM-1 amplifies inflammation and is a crucial mediator of septic shock [J].

Bouchon, A ;

Facchetti, F ;

Weigand, MA ;

Colonna, M .

NATURE, 2001, 410 (6832) :1103-1107

[4] Ventilator associated pneumonia: comparison between quantitative and qualitative cultures of tracheal aspirates [J].

Camargo, LFA ;

De Marco, FV ;

Barbas, CSV ;

Hoelz, C ;

Bueno, MAS ;

Rodrigues, M ;

Amado, VM ;

Caserta, R ;

Martino, MD ;

Pasternak, J ;

Knobel, E .

CRITICAL CARE, 2004, 8 (06) :R422-R430

[5] Ventilator-associated pneumonia [J].

Chastre, J ;

Fagon, JY .

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2002, 165 (07) :867-903

[6] TREM-1 (triggering receptor expressed on myeloid cells): A new player in acute inflammatory responses [J].

Colonna, M ;

Facchetti, F .

JOURNAL OF INFECTIOUS DISEASES, 2003, 187 :S397-S401

[7] STAT1 and STAT3 phosphorylation by porins are independent of JAKs but are dependent on MAPK pathway and plays a role in U937 cells production of interleukin-6 [J].

Galdiero, Marilena ;

Vitiello, Mariateresa ;

D'Isanto, Marina ;

Raieta, Katia ;

Galdiero, Emilia .

CYTOKINE, 2006, 36 (5-6) :218-228

[8] Soluble triggering receptor expressed on myeloid cells 1 as an anti-inflammatory mediator in sepsis [J].

Giamarellos-Bourboulis, EJ ;

Zakynthinos, S ;

Baziaka, F ;

Papadomichelakis, E ;

Virtzili, S ;

Koutoukas, P ;

Armaganidis, A ;

Giamarellou, H ;

Roussos, C .

INTENSIVE CARE MEDICINE, 2006, 32 (02) :237-243

[9] A soluble form of the triggering receptor expressed on myeloid cells-1 modulates the inflammatory response in murine sepsis [J].

Gibot, S ;

Kolopp-Sarda, MN ;

Béné, MC ;

Bollaert, PE ;

Lozniewski, A ;

Mory, F ;

Levy, B ;

Faure, GC .

JOURNAL OF EXPERIMENTAL MEDICINE, 2004, 200 (11) :1419-1426

[10]

KLENSEYTAIT J, 2006, NAT IMMUNOL, V7, P1266

← 1 2 →