A highly sensitive protein-protein interaction assay based on Gaussia luciferase

被引：330

作者：

Remy, Ingrid ^{[1
]}

Michnick, Stephen W. ^{[1
]}

机构：

[1] Univ Montreal, Dept Biochim, Montreal, PQ H3C 3J7, Canada

来源：

NATURE METHODS | 2006年 / 3卷 / 12期

关键词：

D O I：

10.1038/nmeth979

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein-fragment complementation assays (PCAs) provide a general strategy to study the dynamics of protein-protein interactions in vivo and in vitro. The full potential of PCA requires assays that are fully reversible and sensitive at subendogenous protein expression levels. We describe a new assay that meets these criteria, based on the Gaussia princeps luciferase enzyme, demonstrating chemical reversal, and induction and inhibition of a key interaction linking insulin and TGF beta signaling.

引用

页码：977 / 979

页数：3

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