Characterisation of hydrolysable tannins from leaves of Betula pubescens by high-performance liquid chromatography-mass spectrometry

被引：142

作者：

Salminen, JP ^{[1
]}

Ossipov, V

Loponen, J

Haukioja, E

Pihlaja, K

机构：

[1] Turku Univ, Dept Chem, Phys Chem Lab, FIN-20014 Turku, Finland

[2] Turku Univ, Dept Biol, Sect Ecol, FIN-20014 Turku, Finland

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 864卷 / 02期

基金：

芬兰科学院;

关键词：

Betula pubescens; hydrolysable tannins; gallotannins; ellagitannins;

D O I：

10.1016/S0021-9673(99)01036-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatography-electrospray ionisation mass spectrometry (HPLC-ESI-MS) method, assisted by diode array detection, for the characterisation of individual hydrolysable tannins in birch leaves was developed. With the method, it was found that birch (Betula pubescens) leaves contained an exceptionally complex mixture of hydrolysable tannins; 14 gallotannins and 20 ellagitannins were identified. The developed HPLC-ESI-MS method allows the qualitative and quantitative determination of individual gallotannins and ellagitannins directly from crude birch leaf extract. This is important in studying ecological functions of these phenolic compounds, especially their role in the resistance of birch leaves against insects. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：283 / 291

页数：9

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