TFIIS binds to mouse RNA polymerase I and stimulates transcript elongation and hydrolytic cleavage of nascent rRNA

被引:19
作者
Schnapp, G
Graveley, BR
Grummt, I
机构
[1] GERMAN CANC RES CTR, DIV MOL BIOL CELL 2, D-69120 HEIDELBERG, GERMANY
[2] UNIV VERMONT, DEPT MICROBIOL & MOL GENET, MARKEY CTR MOL GENET, BURLINGTON, VT 05405 USA
来源
MOLECULAR & GENERAL GENETICS | 1996年 / 252卷 / 04期
关键词
transcription; RNA polymerase I; elongation; TFIIS;
D O I
10.1007/BF02173006
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Efficient transcription elongation by RNA polymerase I (Pol I) requires a specific Pol I-associated factor, termed TIF-IC. Here we show that TFIIS, a factor that has previously been shown to promote readthrough past many types of blocks to elongation by RNA polymerase II, also enhances Pol I- directed transcription elongation. In a reconstituted transcription system containing purified proteins, TFIIS stimulates Pol I transcription by increasing the overall rate of RNA chain elongation. As with Pol II, ternary Pol I complexes cleave the 3' end of the nascent transcripts in response to TFIIS. The truncated RNAs remain bound to the template, are subject to pyrophosphorolysis, and can be chased into longer transcripts. Moreover, we show by immunoprecipitation and specific affinity chromatography that TFIIS physically interacts with Pol I. The results suggest that nascent transcript cleavage by TFIIS or a TFIIS-related factor may be a general mechanism by which both Pol I and Pol II can bypass transcriptional impediments.
引用
收藏
页码:412 / 419
页数:8
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