High-resolution three-dimensional imaging of red blood cells parasitized by Plasmodium falciparum and in situ hemozoin crystals using optical diffraction tomography

被引：193

作者：

Kim, Kyoohyun ^{[1
]}

Yoon, HyeOk ^{[1
]}

Diez-Silva, Monica ^{[2
]}

Dao, Ming ^{[2
]}

Dasari, Ramachandra R. ^{[3
]}

Park, YongKeun ^{[1
]}

机构：

[1] Korea Adv Inst Sci & Technol, Dept Phys, Taejon 305701, South Korea

[2] MIT, Dept Mat Sci & Engn, Cambridge, MA 02142 USA

[3] MIT, George R Harrison Spect Lab, Cambridge, MA 02194 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2014年 / 19卷 / 01期

基金：

美国国家科学基金会; 新加坡国家研究基金会; 美国国家卫生研究院;

关键词：

red blood cell; malaria; quantitative phase microscopy; digital holographic microscopy; three-dimensional optical imaging; SPECTROSCOPIC PHASE MICROSCOPY; DIGITAL HOLOGRAPHIC MICROSCOPY; MALARIA PARASITE; REFRACTIVE-INDEX; HEMOGLOBIN; ERYTHROCYTES; TRANSPORT; DYNAMICS; VOLUME; LIVE;

D O I：

10.1117/1.JBO.19.1.011005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present high-resolution optical tomographic images of human red blood cells (RBC) parasitized by malaria-inducing Plasmodium falciparum (Pf)-RBCs. Three-dimensional (3-D) refractive index (RI) tomograms are reconstructed by recourse to a diffraction algorithm from multiple two-dimensional holograms with various angles of illumination. These 3-D RI tomograms of Pf-RBCs show cellular and subcellular structures of host RBCs and invaded parasites in fine detail. Full asexual intraerythrocytic stages of parasite maturation (ring to trophozoite to schizont stages) are then systematically investigated using optical diffraction tomography algorithms. These analyses provide quantitative information on the structural and chemical characteristics of individual host Pf-RBCs, parasitophorous vacuole, and cytoplasm. The in situ structural evolution and chemical characteristics of subcellular hemozoin crystals are also elucidated. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)

引用

页数：12

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