Impact of Order and Disorder in RGD Nanopatterns on Cell Adhesion

被引：477

作者：

Huang, Jinghuan ^{[1
,2
,3
]}

Grater, Stefan V. ^{[2
,3
]}

Corbellinl, Francesca ^{[2
,3
]}

Rinck, Sabine ^{[2
,3
]}

Bock, Eva ^{[2
,3
]}

Kemkemer, Ralf ^{[2
,3
]}

Kessler, Horst ^{[4
]}

Ding, Jiandong ^{[1
]}

Spatz, Joachim P. ^{[2
,3
]}

机构：

[1] Fudan Univ, Dept Macromol Sci,Adv Mat Lab, Key Lab Mol Engn Polymers, Minist Educ, Shanghai 200433, Peoples R China

[2] Univ Heidelberg, Max Planck Inst Met Res, Dept New Mat & Biosyst, D-70569 Stuttgart, Germany

[3] Univ Heidelberg, Dept Biophys Chem, D-70569 Stuttgart, Germany

[4] Tech Univ Munich, Dept Chem, Ctr Integrated Prot Sci Munich, D-85747 Garching, Germany

来源：

NANO LETTERS | 2009年 / 9卷 / 03期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

INTEGRIN LIGANDS; PEPTIDES; INTERFACES; SURFACES; PROLIFERATION; ORGANIZATION; FIBROBLASTS; COPOLYMERS; MIGRATION; BINDING;

D O I：

10.1021/nl803548b

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

We herein present a novel platform of well-controlled ordered and disordered nanopatterns positioned with a cyclic peptide of arginine-glycine-aspartic acid (RGD) on a bioinert poly(ethylene glycol) background, to study whether the nanoscopic order of spatial patterning of the integrin-specific ligands influences osteoblast adhesion. This is the first time that the nanoscale order of RGD ligand patterns was varied quantitatively, and tested for its impact on the adhesion of tissue cells. Our findings reveal that Integrin clustering and such adhesion induced by RGD ligands Is dependent on the local order of ligand arrangement on a substrate when the global average ligand spacing is larger than 70 nm; i.e., cell adhesion Is "turned off" by RGD nanopattern order and "turned on" by the RGD nanopattern disorder if operating at this range of interligand spacing.

引用

页码：1111 / 1116

页数：6

共 35 条

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Activation of integrin function by nanopatterned adhesive interfaces [J].