Evaluation of donor skin disinfection methods

被引:74
作者
Goldman, M [1 ]
Roy, G [1 ]
Frechette, N [1 ]
Decary, F [1 ]
Massicotte, L [1 ]
Delage, G [1 ]
机构
[1] LAB SANTE PUBL QUEBEC, MONTREAL, PQ, CANADA
关键词
D O I
10.1046/j.1537-2995.1997.37397240214.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
BACKGROUND: Because most bacteria isolated from contaminated platelet concentrates are thought to originate from the donor's skin, the efficacy of four methods of skin disinfection was compared. STUDY DESIGN AND METHODS: Contact plates were used for antecubital skin cultures after they were demonstrated to be easier to use and at least as sensitive as a swab system. One antecubital fossa of each subject was disinfected by a standard method, the use of a povidone-iodine swabstick containing 0.75-percent available iodine followed by the use of a povidone-iodine swabstick containing I-percent available iodine. The other arm was disinfected with either a 70-percent isopropyl alcohol scrub followed by an ampoule of 2-percent iodine tincture (Group 1, n = 126); a green-soap sponge followed by a 70-percent isopropyl alcohol swab, used for donors who are allergic to iodine (Group 2; n = 30); or a 0.5-percent chlorhexidine gluconate and 70-percent isopropyl alcohol sponge followed by an ampoule of 0.5-percent chlorhexidine gluconate and 70-percent isopropyl alcohol (Group 3; n = 40). Contact plate cultures were done before and after disinfection, and colonies counted after a 48-hour 37 degrees C incubation period. RESULTS: Similar numbers of bacteria grew from both antecubital fossae of the same subject before disinfection (p = 0.71). Compared to the standard povidone-iodine method, isopropyl alcohol and tincture of iodine resulted in significantly less bacterial growth (p<0.001), the green soap and isopropyl alcohol method resulted in significantly more bacterial growth (p<0.001), and the chlorhexidine gluconate and isopropyl alcohol method resulted in similar amounts of bacterial growth (p>0.3). CONCLUSION: Isopropyl alcohol scrub followed by iodine tincture is more efficacious than povidone-iodine as measured by contact plate cultures. For donors who are allergic to iodine, chlorhexidine gluconate and isopropyl alcohol is more efficacious than green soap and isopropyl alcohol.
引用
收藏
页码:309 / 312
页数:4
相关论文
共 15 条
[1]   TRANSFUSION-RELATED SEPSIS AFTER PROLONGED PLATELET STORAGE [J].
ANDERSON, KC ;
LEW, MA ;
GORGONE, BC ;
MARTEL, J ;
LEAMY, CB ;
SULLIVAN, B .
AMERICAN JOURNAL OF MEDICINE, 1986, 81 (03) :405-411
[2]  
BLAJCHMAN MA, 1994, AM SOC HEMATOL S1, V84, pA529
[3]  
BRUCH MK, 1991, DISINFECTION STERILI, P1028
[4]  
GOLDMAN M, 1991, Transfusion Medicine Reviews, V5, P73, DOI 10.1016/S0887-7963(91)70194-6
[5]  
KITCHEN AD, 1995, TRANSFUS MED S, V5, P50
[6]   PROSPECTIVE RANDOMIZED TRIAL OF POVIDONE-IODINE, ALCOHOL, AND CHLORHEXIDINE FOR PREVENTION OF INFECTION ASSOCIATED WITH CENTRAL VENOUS AND ARTERIAL CATHETERS [J].
MAKI, DG ;
RINGER, M ;
ALVARADO, CJ .
LANCET, 1991, 338 (8763) :339-343
[7]  
MORROW J F, 1991, Journal of the American Medical Association, V266, P555, DOI 10.1001/jama.266.4.555
[8]  
PLEASANT H, 1994, TRANSFUSION S, V34
[9]  
RUSSELL AD, 1992, PRINCIPLES PRACTICE, P310
[10]   THE EFFECT OF SKIN DISINFECTION MATERIALS ON REDUCING BLOOD CULTURE CONTAMINATION [J].
SCHIFMAN, RB ;
PINDUR, A .
AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1993, 99 (05) :536-538