A hypermorphic mutation in the protein phosphatase 2C HAB1 strongly affects ABA signaling in Arabidopsis

被引：76

作者：

Robert, Nadia

Merlot, Sylvain

N'Guyen, Vinh

Boisson-Dernier, Aurelien

Schroeder, Julian I.

机构：

[1] Univ Calif San Diego, Ctr Mol Genet, Div Biol Sci, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Ctr Mol Genet, Cell & Dev Biol Sect, La Jolla, CA 92093 USA

[3] CNRS, Inst Sci Vegetal, UPR 2355, F-91198 Gif Sur Yvette, France

来源：

FEBS LETTERS | 2006年 / 580卷 / 19期

关键词：

protein phosphatase 2C; abscisic acid; dominant mutation; hypermorphic;

D O I：

10.1016/j.febslet.2006.07.047

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein phosphatases of the 2C family (PP2C) function in the regulation of several signaling pathways from prokaryotes to eukaryotes. In Arabidopsis thaliana, the HAB1 PP2C is a negative regulator of the stress hormone abscisic acid (ABA) signaling. Here, we show that plants expressing a mutant form of HAB1 in which Gly246 was mutated to Asp (G246D) display strong ABA insensitive phenotypes. Our results indicate that the G246D mutation has a hypermorphic rather than a dominant negative effect. The data suggest that this mutation localized in a conserved motif in the PP2C catalytic domain could be used in other PP2Cs to reveal their biological functions. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：4691 / 4696

页数：6

共 28 条

[1] Spalten, a protein containing Gα-protein-like and PP2C domains, is essential for cell-type differentiation in Dictyostelium [J].