Rapid screening for anthocyanins and anthocyanin dimers in crude grape extracts by high performance liquid chromatography coupled with diode array detection and tandem mass spectrometry

被引：54

作者：

Pati, Sandra ^{[1
]}

Liberatore, Maria Teresa ^{[2
]}

Gambacorta, Giuseppe ^{[1
]}

Antonacci, Donato ^{[3
]}

La Notte, Ennio ^{[1
]}

机构：

[1] Univ Foggia, Dept Food Sci, Foggia, Italy

[2] Univ Foggia, Prod Sci Engn & Econ Agr Syst Dept, Fac Agr, I-71100 Foggia, Italy

[3] CRA UTV, Unita Ric Uva Tavola & Vitivinicoltura Ambiente M, I-70010 Turi, BA, Italy

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 18期

关键词：

Anthocyanin; Dimers; Tandem mass spectrometry; Rapid resolution; High performance liquid chromatography; Grape; RED WINE; HPLC; IDENTIFICATION; ELECTROSPRAY; COLORANTS; STABILITY; CYANIDIN; JUICES; COLUMN; FOOD;

D O I：

10.1016/j.chroma.2009.02.068

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

A rapid and efficient method using high performance liquid chromatography coupled with diode array detection and tandem mass spectrometry (HPLC-DAD-MS/MS) for fast screening large numbers of anthocyanins and anthocyanin dimers in different grape skin extracts, without further sample cleanup procedures, was developed. A good separation of most detected anthocyanins was achieved in a run time of 15 min. identification of anthocyanin pigments required a combination of several information: UV-vis spectra, MS and MS/MS spectra, and elution pattern. Many compounds have been here detected for the first time and their structures tentatively elucidated. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：3864 / 3868

页数：5

共 28 条

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Cyanidin and cyanidin 3-O-β-D-glucoside as DNA cleavage protectors and antioxidants [J].