Effect of ultrasonic treatment on the biochemphysical properties of chitosan

被引:105
作者
Liu, Hui [1 ]
Bao, Jianguo
Du, Yumin
Zhou, Xuan
Kennedy, John F.
机构
[1] China Univ Geosci, Sch Environm Studies, Wuhan 430074, Peoples R China
[2] China Univ Geosci, Key Lab Biol & Environm Geol, Wuhan 430074, Peoples R China
[3] Wuhan Univ, Dept Environm Sci, Wuhan 430072, Peoples R China
[4] Univ Birmingham, Sch Chem Sci, Carbohydrate & Prot Technol Grp, Birmingham B15 2TT, W Midlands, England
[5] Univ Birmingham Res Pk, Chembiotech Labs, Birmingham B15 2SQ, W Midlands, England
基金
中国国家自然科学基金;
关键词
chitosan; ultrasonic; molecular weight; deacetylation degree; antimicrobial activity;
D O I
10.1016/j.carbpol.2005.11.007
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Four chitosans with different molecular weights and degrees of deacetylation degree and 28 chitosans derived from these initial chitosans by ultrasonic degradation have been characterized by gel permeation chromatography (GPC), FT-IR spectroscopy, X-ray diffraction and titrimetric analyses. Antimicrobial activities were investigated against E. coli and S. aureus using an inhibitory rate technique. The results showed that ultrasonic treatment decreased the molecular weight of chitosan, and that chitosan with higher molecular weight and higher DID was more easily degraded. The polydispersity decreased with ultrasonic treatment time, which was in linear relationship with the decrease of molecular weight. Ultrasonic degradation changed the DD of initial chitosan with a lower DD (< 90%), but not the DD of the initials chitosan with a higher DID (> 90%). The increased crystallinity of ultrasonically treated chitosan indicated that ultrasonic treatment changed the physical structure of chitosan, mainly due to the decrease of molecular weight. Ultrasonic treatment enhanced the antimicrobial activity of chitosan, mainly due to the decrease of molecular weight. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:553 / 559
页数:7
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