Assessment of IgG antibodies against yellow fever virus after vaccination with 17D by different assays: neutralization test, haemagglutination inhibition test, immunofluorescence assay and ELISA

被引：101

作者：

Niedrig, M

Lademann, M

Emmerich, P

Lafrenz, M

机构：

[1] Robert Koch Inst, D-13353 Berlin, Germany

[2] Univ Rostock, Klin & Poliklin Innere Med, Fak Med, D-2500 Rostock 1, Germany

[3] Bernhard Nocht Inst Trop Med, Hamburg, Germany

来源：

TROPICAL MEDICINE & INTERNATIONAL HEALTH | 1999年 / 4卷 / 12期

关键词：

17D yellow fever vaccination; antibody persistence; YF neutralization test; immunofluorescence; assay; haemagglutination inhibition test; ELISA;

D O I：

10.1046/j.1365-3156.1999.00496.x

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

We analysed serum samples of 209 subjects immunized with yellow fever vaccine 17D by different assays: neutralization test, immunofluorescence assay, haemagglutination inhibition test and ELISA, for presence of 17D-specific antibodies. Serum samples were taken from a few weeks up to 35 years after vaccination. The neutralization test had the highest sensitivity. There was no correlation of results between the serological assays. Considering NT titres > 1:10 as indicating protection, we found that about 75% of subjects remained immune even 10 years after vaccination, with a median NT titre of 1:40 in reactive sera.

引用

页码：867 / 871

页数：5

共 18 条

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