Cloning and characterization of a rhoGAP homolog from Dictyostelium discoideum

Small GTPases interact with a variety of proteins that affect nucleotide binding and cleavage. GTPase activating proteins (GAPs) art: one class of these proteins that act by accelerating ire intrinsic GTPase rare resulting in the formation of the biologically inactive GDP-bound farm of the GTPase, For the Rho subfamily of GTPases, there is a growing number of proteins with rhoGAP activity that are identifiable by a homologous region of about 150 amino acids, We have exploited this homology using the polymerase chain reaction to clone the first rhoGAP homolog, called DdRacGAP, from the slime mold Dictyostelium discoideum. The GAP domain of DdRacGAP (amino acids 1-212), when expressed and purified from Escherichia coli, is active on both Dictyostelium and human Rho Family GTPases but not human Ras. The full-length protein is 1356 amino acids in length and has several interesting homologies ill addition to the GAP domain, including an SH3 domain, a dbl homology domain, and a pleckstrin homology domain.