Serum resistance, an important virulence determinant of Borrelia burgdorferi sensu lato strains belonging to the Borrelia afzelii and B. burgdorferi sensu stricto genotypes, is related to binding of the complement inhibitor factor H to the spirochete surface protein outer surface protein E (OspE) and its homologues. In this study, we show that the C-terminal short consensus repeats 18-20 of both human and mouse factor H bind to OspE. Analogously, factor H-related protein 1, a distinct plasma protein with three short consensus repeat domains homologous to those in factor H, bound to OspE. Deleting 15-aa residues (region V) from the C terminus of the OspE paralog P21 (a 20.7-kDa OspE-paralogous surface lipoprotein in the B. burgdorferi sensu stricto 297 strain) abolished factor H binding. However, C-terminal peptides from OspE, P21, or OspEF-related protein P alone and the C-terminal deletion mutants of P21 inhibited factor H binding to OspE only partially when compared with full-length P21 or its N-terminal mutant. Alanine substitution of amino acids in peptides from the key binding regions of the OspE family indicated that several lysine residues are required for factor H binding. Thus, the borrelial OspE family proteins bind the C inhibitor factor H via multiple sites in a lysine-dependent manner. The C-terminal site V (Ala(151)-Lys(166)) is necessary, but not sufficient, for factor H binding in both rodents and humans. Identification of the necessary binding sites forms a basis for the development of vaccines that block the factor H-OspE interaction and thereby promote the killing of Borreliae.
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Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USAUniv Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Casjens, S
Palmer, N
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Palmer, N
van Vugt, R
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
van Vugt, R
Huang, WM
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Huang, WM
Stevenson, B
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Stevenson, B
Rosa, P
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Rosa, P
Lathigra, R
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Lathigra, R
Sutton, G
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Sutton, G
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Peterson, J
Dodson, RJ
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Dodson, RJ
Haft, D
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Haft, D
Hickey, E
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Hickey, E
Gwinn, M
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Gwinn, M
White, O
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
White, O
Fraser, CM
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
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Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USAUniv Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Casjens, S
Palmer, N
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Palmer, N
van Vugt, R
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
van Vugt, R
Huang, WM
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Huang, WM
Stevenson, B
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Stevenson, B
Rosa, P
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Rosa, P
Lathigra, R
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Lathigra, R
Sutton, G
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Sutton, G
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Peterson, J
Dodson, RJ
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Dodson, RJ
Haft, D
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Haft, D
Hickey, E
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Hickey, E
Gwinn, M
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机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
Gwinn, M
White, O
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h-index: 0
机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA
White, O
Fraser, CM
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h-index: 0
机构:Univ Utah, Sch Med, Dept Oncol Sci, Div Mol Biol & Genet, Salt Lake City, UT 84132 USA