A novel von Willebrand factor binding protein expressed by Staphylococcus aureus

被引:77
作者
Bjerketorp, J
Nilsson, M
Ljungh, Å
Flock, JI
Jacobsson, K
Frykberg, L
机构
[1] Swedish Univ Agr Sci, Dept Microbiol, SE-75007 Uppsala, Sweden
[2] Lund Univ, Dept Med Microbiol & Infect Dis, SE-22362 Lund, Sweden
[3] Huddinge Univ Hosp, Karolinska Inst, Dept Microbiol Pathol & Immunol, SE-14186 Huddinge, Sweden
来源
MICROBIOLOGY-SGM | 2002年 / 148卷
关键词
receptin; virulence factor; phage display;
D O I
10.1099/00221287-148-7-2037
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
When a shotgun phage-display library of Staphylococcus aureus Newman was affinity selected (panned) against recombinant von Willebrand factor (vWf), a novel von Willebrand factor binding protein (vWbp) was found. Experimental data indicate that the interaction between vWbp and vWf is very specific and mediated by a region of 26 aa residues in the C-terminal part of vWbp. vWbp has an N-terminal secretory signal sequence but no cell wall anchoring motif, suggesting a soluble extracellular location. Mature vWbp could be purified from the culture supernatant and the identity of the protein was confirmed by N-terminal sequencing. vWbp migrates with an apparent molecular mass of 66 kDa and the deduced protein consists of 482 aa. The gene encoding vWbp, named vwb, was present in all S. aureus strains investigated.
引用
收藏
页码:2037 / 2044
页数:8
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