Increase of transcriptional levels of egr-1 and nur77 genes due to both nicotine treatment and withdrawal in pheochromocytoma cells

被引：12

作者：

Ichino, N ^{[1
]}

Yamada, K ^{[1
]}

Nishii, K ^{[1
]}

Sawada, H ^{[1
]}

Nagatsu, T ^{[1
]}

Ishiguro, H ^{[1
]}

机构：

[1] Fujita Hlth Univ, Inst Comprehens Med Sci, Toyoake, Aichi 4701192, Japan

来源：

JOURNAL OF NEURAL TRANSMISSION | 2002年 / 109卷 / 7-8期

关键词：

PC12; cells; nicotine; immediate early genes; egr-1; nur77; withdrawal;

D O I：

10.1007/s007020200084

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

The influence of nicotine on the expression of egr-1 and nur77 genes by nicotine treatment and withdrawal was assessed using PC12 cells. Nicotine treatment significantly increased the amount of mRNA for egr-1 and nur77 genes at 0.5 h post-nicotine treatment in the PC12 cells. In addition, nicotine withdrawal also elevated transcriptional levels of egr-1 and nur77 genes in Northern blot analyses. Nicotine treatment (200 muM) was also found to significantly increase expressional levels of Egr-1 and Nur77 proteins at 0.5 h post-nicotine treatment. In contrast, Egr-1 and Nur77 protein levels were dramatically decreased by nicotine withdrawal. These results suggest that expressional levels of Egr-1 and Nur77 proteins in neural cells may affect the transcriptional activity of late-response genes after nicotine withdrawal.

引用

页码：1015 / 1022

页数：8

共 26 条

[1]

BADLEY JE, 1988, BIOTECHNIQUES, V6, P114

[2]

THE MULTIFUNCTIONAL CALCIUM CALMODULIN-DEPENDENT PROTEIN-KINASE - FROM FORM TO FUNCTION [J].

BRAUN, AP ;

SCHULMAN, H .

ANNUAL REVIEW OF PHYSIOLOGY, 1995, 57 :417-445

[3]

FUNCTIONAL DOMAINS AND PHOSPHORYLATION OF THE ORPHAN RECEPTOR NUR77 [J].

DAVIS, IJ ;

HAZEL, TG ;

CHEN, RH ;

BLENIS, J ;

LAU, LF .

MOLECULAR ENDOCRINOLOGY, 1993, 7 (08) :953-964

[4]

LASER-INITIATED POLYMERIZATION [J].

DAVIS, TP .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 1994, 77 (01) :1-7

[5]

ACCURATE TRANSCRIPTION INITIATION BY RNA POLYMERASE-II IN A SOLUBLE EXTRACT FROM ISOLATED MAMMALIAN NUCLEI [J].

DIGNAM, JD ;

LEBOVITZ, RM ;

ROEDER, RG .

NUCLEIC ACIDS RESEARCH, 1983, 11 (05) :1475-1489

[6]

EBERT SN, 1994, J BIOL CHEM, V269, P20885

[7]

GRABCZEWSKA E, 1990, INT J TISSUE REACT, V12, P281

[8]

STIMULATION OF NEURONAL ACETYLCHOLINE-RECEPTORS INDUCES RAPID GENE-TRANSCRIPTION [J].

GREENBERG, ME ;

ZIFF, EB ;

GREENE, LA .

SCIENCE, 1986, 234 (4772) :80-83

[9]

NUR77 IS DIFFERENTIALLY MODIFIED IN PC12 CELLS UPON MEMBRANE DEPOLARIZATION AND GROWTH-FACTOR TREATMENT [J].

HAZEL, TG ;

MISRA, R ;

DAVIS, IJ ;

GREENBERG, ME ;

LAU, LF .

MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (06) :3239-3246

[10]

HIREMAGALUR B, 1993, J BIOL CHEM, V268, P23704

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