Development of microsatellite markers in black locust (Robinia pseudoacacia) using a dual-supression-PCR technique

被引：142

作者：

Lian, C ^{[1
]}

Hogetsu, T ^{[1
]}

机构：

[1] Univ Tokyo, Asian Nat Environm Sci Ctr, Tokyo 1880002, Japan

来源：

MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 03期

关键词：

(AC)(10) primer; black locust; dual-suppression-PCR technique; microsatellite; Robinia pseudoacacia;

D O I：

10.1046/j.1471-8278.2002.00213.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Black locust (Robinia pseudoacacia) is an economically and ecologically important tree species in the world. We isolated seven polymorphic microsatellite loci from R. pseudoacacia using a dual-suppression-PCR technique. These loci provided microsatellite markers with high polymorphism ranging from three to 12 alleles per locus and expected heterozygosity between 0.538 and 0.944. The markers are now available for more detailed investigation of population genetic structure and pollen and seed dispersal.

引用

页码：211 / 213

页数：3

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