Visualization of plant cell walls by atomic force microscopy

被引：105

作者：

Kirby, AR

Gunning, AP

Waldron, KW

Morris, VJ

Ng, A

机构：

[1] Institute of Food Research, Norwich Laboratory, Colney, Norwich, NR4 7UA, Norwich Research Park

[2] Institute of Food Research, Colney, Norwich NR4 7UA, Norwich Research Park

来源：

BIOPHYSICAL JOURNAL | 1996年 / 70卷 / 03期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1016/S0006-3495(96)79708-4

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Atomic force microscopy has been used to visualize the ultrastructure of hydrated plant cell wall material from prepared apple (Malus pumila MILL; Cox orange pippin), water chestnut (Eleocharis dulcis L.), potato (Solanum tuberosum L.; Bintje), and carrot (Daucus carota L.; Amsterdamse bak) parenchyma. Samples of cell wall material in aqueous suspension were deposited onto freshly cleaved mica. Excess water was blotted away and the moist samples were imaged in air at ambient temperature and humidity. The three-dimensional images obtained highlighted the layered structure of the plant cell walls and revealed features interpreted as individual cellulose microfibrils and plasmodesmata.

引用

页码：1138 / 1143

页数：6

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