Towards a CRISPR view of early human development: applications, limitations and ethical concerns of genome editing in human embryos

被引：42

作者：

Reyes, Alvaro Plaza

Lanner, Fredrik ^{[1
]}

机构：

[1] Karolinska Univ Sjukhuset, Karolinska Inst, Dept Clin Sci Intervent & Technol, S-14186 Stockholm, Sweden

来源：

DEVELOPMENT | 2017年 / 144卷 / 01期

关键词：

CRISPR; Embryo; Genome editing; Ethics; SEQUENCE-SPECIFIC CONTROL; DOUBLE-STRAND BREAKS; HALOFERAX-MEDITERRANEI; SELF-ORGANIZATION; CELL LINEAGES; RNA; MOUSE; CAS9; RECOMBINATION; TRANSCRIPTION;

D O I：

10.1242/dev.139683

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Developmental biologists have become increasingly aware that the wealth of knowledge generated through genetic studies of preimplantation mouse development might not easily be translated to the human embryo. Comparative studies have been fueled by recent technological advances in single-cell analysis, allowing in-depth analysis of the human embryo. This field could shortly gain more momentum as novel genome editing technologies might, for the first time, also allow functional genetic studies in the human embryo. In this Spotlight article, we summarize the CRISPR-Cas9 genome editing system and discuss its potential applications and limitations in human pre-implantation embryos, and the ethical considerations thereof.

引用

页码：3 / 7

页数：5

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