Differential mobilization of tyrosine kinases in human platelets stimulated with thrombin or thrombin receptor agonist peptide

被引：18

作者：

Satoh, K ^{[1
]}

Ozaki, Y ^{[1
]}

Asazuma, N ^{[1
]}

Yatomi, Y ^{[1
]}

Qi, RM ^{[1
]}

Kuroda, K ^{[1
]}

Yang, LB ^{[1
]}

Kume, S ^{[1
]}

机构：

[1] YAMANASHI MED UNIV,DEPT CLIN LAB & MED,YAMANASHI 40938,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 225卷 / 03期

关键词：

D O I：

10.1006/bbrc.1996.1299

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Both thrombin and thrombin receptor agonist peptide (TRAP) activated p72(syk) and p60(c-src) with similar magnitudes. Both thrombin and TRAP induced translocation of p60(c-src) and p54/58(lyn) to cytoskeleton in an aggreagation-dependent manner. Thrombin also induced cytoskeletal association of p72(syk), but independent of platelet aggregation. Furthermore, p72(syk) associated with cytoskeleton underwent marked proteolysis, which was partially dependent upon calpain activation. In contrast, TRAP, even at concentrations as high as 100 mu M, did not induce p72(syk) translocation to cytoskeleton. Our findings suggest that cytoskeletal translocation of p72(syk) induced by thrombin is governed by a mechanism distinct from those of p60(c-src) and p54/58(lyn) translocation. It is also suggested that p72(syk) translocation induced by thrombin requires additional signal(s) other than that mediated by the recently-cloned thrombin receptor that couples with GTP-binding proteins and interacts with TRAP. (C) 1996 Academic Press. Inc.

引用

页码：1084 / 1089

页数：6

共 24 条

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