Construction of mini-Tn10luxABcam/Ptac-ATS and its use for developing a bacteriophage that transduces bioluminescence to Escherichia coli O157:H7

被引：38

作者：

Waddell, TE ^{[1
]}

Poppe, C ^{[1
]}

机构：

[1] Hlth Canada, Hlth Protect Branch, Guelph Lab, Guelph, ON N1G 2W1, Canada

来源：

FEMS MICROBIOLOGY LETTERS | 2000年 / 182卷 / 02期

关键词：

Escherichia coli O157 : H7; bacteriophage; bioluminescence;

D O I：

10.1111/j.1574-6968.2000.tb08909.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mini-Tn10luxABcam/Ptac-ATS was constructed in order to develop a luciferase-transducing bacteriophage for detecting Escherichia coli O157:H7. The transposon was designed to deliver a 3.6-kb insertion that confers n-decanal-dependent bioluminescence and resistance to chroramphenicol and was constructed using mini-Tn10cam/Ptac-ATS in the plasmid pNK2884 and luxAB from Vibrio harveyi. Phi V10, a temperate bacteriophage infecting common phage types of Escherichia coli O157:H7, was mutagenized as a prophage in E. coli O157:H7 strain R508. Phi V10::lux-ABcamA1-23 was rescued from the strain by propagating it on a strain lacking the bacteriophage and the vector containing the transposon. The bacteriophage transduced n-decanal-dependent bioluminescence to E. coli O157:H7 strain R508 that was measurable approximately 1 h post infection. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：285 / 289

页数：5

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