Lateral diffusion of Toll-like receptors reveals that they are transiently confined within lipid rafts on the plasma membrane

被引:122
作者
Triantafilou, M
Morath, S
Mackie, A
Hartung, T
Triantafilou, K [1 ]
机构
[1] Univ Sussex, Infect & Immun Grp, Sch Life Sci, Brighton BN1 9QG, E Sussex, England
[2] Univ Konstanz, Dept Biochem Pharmacol, D-78457 Constance, Germany
[3] Inst Food Res, Dept Food Biophys, Norwich NR4 7UA, Norfolk, England
关键词
LTA; toll-like receptors; LPS-activation cluster; innate recognition; FRAP;
D O I
10.1242/jcs.01270
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The innate immune system utilises pattern recognition receptors in order to recognise microbial conserved molecular patterns. The family of Toll-like receptors (TLRs) has been shown to act as the main pattern recognition receptors for the innate immune system. Using biochemical as well as fluorescence imaging techniques, TLR2 and TLR4 were found to be recruited within microdomains upon stimulation by bacterial products. Furthermore their lateral diffusion in the cell membrane as determined by fluorescence recovery after photobleaching revealed that upon stimulation by bacterial products TLRs encounter barriers to their lateral movement, thus supporting the notion that specialised domains on the plasma membrane facilitate the innate recognition.
引用
收藏
页码:4007 / 4014
页数:8
相关论文
共 45 条
[1]   Recognition of double-stranded RNA and activation of NF-κB by Toll-like receptor 3 [J].
Alexopoulou, L ;
Holt, AC ;
Medzhitov, R ;
Flavell, RA .
NATURE, 2001, 413 (6857) :732-738
[2]   SORTING OF GPI-ANCHORED PROTEINS TO GLYCOLIPID-ENRICHED MEMBRANE SUBDOMAINS DURING TRANSPORT TO THE APICAL CELL-SURFACE [J].
BROWN, DA ;
ROSE, JK .
CELL, 1992, 68 (03) :533-544
[3]   Heat shock protein 90 mediates macrophage activation by Taxol and bacterial lipopolysaccharide [J].
Byrd, CA ;
Bornmann, W ;
Erdjument-Bromage, H ;
Tempst, P ;
Pavletich, N ;
Rosen, N ;
Nathan, CF ;
Ding, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (10) :5645-5650
[4]  
Delude RL, 1998, J IMMUNOL, V161, P3001
[5]   DIFFERENCES BETWEEN THE LATERAL ORGANIZATION OF CONVENTIONAL AND INOSITOL PHOSPHOLIPID-ANCHORED MEMBRANE-PROTEINS - A FURTHER DEFINITION OF MICROMETER SCALE MEMBRANE DOMAINS [J].
EDIDIN, M ;
STROYNOWSKI, I .
JOURNAL OF CELL BIOLOGY, 1991, 112 (06) :1143-1150
[6]   Shrinking patches and slippery rafts: scales of domains in the plasma membrane [J].
Edidin, M .
TRENDS IN CELL BIOLOGY, 2001, 11 (12) :492-496
[7]   Microdomains of GPI-anchored proteins in living cells revealed by crosslinking [J].
Friedrichson, T ;
Kurzchalia, TV .
NATURE, 1998, 394 (6695) :802-805
[8]   Lipid domain structure of the plasma membrane revealed by patching of membrane components [J].
Harder, T ;
Scheiffele, P ;
Verkade, P ;
Simons, K .
JOURNAL OF CELL BIOLOGY, 1998, 141 (04) :929-942
[9]   Caveolae, DIGs, and the dynamics of sphingolipid-cholesterol microdomains [J].
Harder, T ;
Simons, K .
CURRENT OPINION IN CELL BIOLOGY, 1997, 9 (04) :534-542
[10]   The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5 [J].
Hayashi, F ;
Smith, KD ;
Ozinsky, A ;
Hawn, TR ;
Yi, EC ;
Goodlett, DR ;
Eng, JK ;
Akira, S ;
Underhill, DM ;
Aderem, A .
NATURE, 2001, 410 (6832) :1099-1103