Synthesis of duplex DNA containing a spin labeled analog of 2'-deoxycytidine

被引：12

作者：

Fischhaber, PL ^{[1
]}

Reese, AW ^{[1
]}

Nguyen, T ^{[1
]}

Kirchner, JJ ^{[1
]}

Hustedt, EJ ^{[1
]}

Robinson, BH ^{[1
]}

Hopkins, PB ^{[1
]}

机构：

[1] UNIV WASHINGTON,DEPT CHEM,SEATTLE,WA 98195

来源：

NUCLEOSIDES & NUCLEOTIDES | 1997年 / 16卷 / 04期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1080/07328319708001356

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the chemical synthesis of phosphoramidite 8, containing a spin labelled analog of deoxycytidine, C*, and its incorporation into synthetic DNA. The EPR characteristics of the resulting DNAs indicated that the motion of the spin label was well-correlated with the uniform modes of the macromolecule, but that correlation of the spin label with internal motion was less effective than that achieved using a spin labelled quinolone, Q.

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页码：365 / 377

页数：13

相关论文

共 11 条

[1] OLIGODEOXYNUCLEOTIDES CONTAINING C-5 PROPYNE ANALOGS OF 2'-DEOXYURIDINE AND 2'-DEOXYCYTIDINE [J].

FROEHLER, BC ;

WADWANI, S ;

TERHORST, TJ ;

GERRARD, SR .

TETRAHEDRON LETTERS, 1992, 33 (37) :5307-5310

[2] PALLADIUM-CATALYZED SYNTHESIS OF ALKYNYLAMINO NUCLEOSIDES - A UNIVERSAL LINKER FOR NUCLEIC-ACIDS [J].

HOBBS, FW .

JOURNAL OF ORGANIC CHEMISTRY, 1989, 54 (14) :3420-3422

[3] MONITORING DNA DYNAMICS USING SPIN-LABELS WITH DIFFERENT INDEPENDENT MOBILITIES [J].

HUSTEDT, EJ ;

KIRCHNER, JJ ;

SPALTENSTEIN, A ;

HOPKINS, PB ;

ROBINSON, BH .

BIOCHEMISTRY, 1995, 34 (13) :4369-4375

[4] MOTIONS OF SHORT DNA DUPLEXES - AN ANALYSIS OF DNA DYNAMICS USING AN EPR-ACTIVE PROBE [J].

HUSTEDT, EJ ;

SPALTENSTEIN, A ;

KIRCHNER, JJ ;

HOPKINS, PB ;

ROBINSON, BH .

BIOCHEMISTRY, 1993, 32 (07) :1774-1787

[5] DNA DYNAMICS FROM A SPIN PROBE - DEPENDENCE OF PROBE MOTION ON TETHER LENGTH [J].

KIRCHNER, JJ ;

HUSTEDT, EJ ;

ROBINSON, BH ;

HOPKINS, PB .

TETRAHEDRON LETTERS, 1990, 31 (05) :593-596

[6] A PROBE FOR SEQUENCE-DEPENDENT NUCLEIC-ACID DYNAMICS [J].

MILLER, TR ;

ALLEY, SC ;

REESE, AW ;

SOLOMON, MS ;

MCCALLISTER, WV ;

MAILER, C ;

ROBINSON, BH ;

HOPKINS, PB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (36) :9377-9378

[7] DNA STRUCTURAL DATA FROM A DYNAMICS PROBE - THE DYNAMIC SIGNATURES OF SINGLE-STRANDED, HAIRPIN-LOOPED, AND DUPLEX FORMS OF DNA ARE DISTINGUISHABLE [J].

SPALTENSTEIN, A ;

ROBINSON, BH ;

HOPKINS, PB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (06) :2303-2305

[8] SEQUENCE-DEPENDENT AND STRUCTURE-DEPENDENT DNA-BASE DYNAMICS - SYNTHESIS, STRUCTURE, AND DYNAMICS OF SITE AND SEQUENCE SPECIFICALLY SPIN-LABELED DNA [J].

SPALTENSTEIN, A ;

ROBINSON, BH ;

HOPKINS, PB .

BIOCHEMISTRY, 1989, 28 (24) :9484-9495

[9] STRUCTURAL STUDIES OF PROTEIN NUCLEIC-ACID INTERACTION - THE SOURCES OF SEQUENCE-SPECIFIC BINDING [J].

STEITZ, TA .

QUARTERLY REVIEWS OF BIOPHYSICS, 1990, 23 (03) :205-+

[10] TRANSIENT PROTECTION - EFFICIENT ONE-FLASK SYNTHESES OF PROTECTED DEOXYNUCLEOSIDES [J].

TI, GS ;

GAFFNEY, BL ;

JONES, RA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1982, 104 (05) :1316-1319