The high-speed Hydra-Plus-One system for automated high-throughput protein crystallography

被引：38

作者：

Krupka, HI

Rupp, B ^{[1
]}

Segelke, BW

Lekin, TP

Wright, D

Wu, HC

Todd, P

Azarani, A

机构：

[1] Lawrence Livermore Natl Lab, Macromol Crystallog & TB Struct Genom Consortium, Livermore, CA 94551 USA

[2] Robbins Sci Corp, Sunnyvale, CA 94089 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2002年 / 58卷

关键词：

automation; crystallization; high-throughput; Hydra (R); liquid-handling system;

D O I：

10.1107/S090744490201435X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An automated high-throughput dispenser has been developed for the setup of protein crystallization trials by vapor diffusion or Microbatch methods. The Hydra(R)-Plus-One is composed of a Hydra(R)-PP system equipped with a motorized XYZ-platform, 96 precision glass syringes and a single-channel microsolenoid dispenser, which transfers 100nl-50mul of protein solution with an accuracy of > 90% at a speed of 60s per 96 wells. Up to 300mul of premixed cocktails can be aspirated with the 96-syringe-assembly and dispensed into reservoir and droplet wells within 60s. The Hydra(R)-Plus-One combines high precision, reliability and speed in a cost- effective high-throughput system ideally suited for protein crystallization.

引用

页码：1523 / 1526

页数：4

共 6 条

[1] Intelligent decision support for protein crystal growth [J].