DNA methylation and chromosome instability in breast cancer cell lines

被引：33

作者：

Vilain, A ^{[1
]}

Vogt, N ^{[1
]}

Dutrillaux, B ^{[1
]}

Malfoy, B ^{[1
]}

机构：

[1] Inst Curie, CNRS, UMR 147, F-75248 Paris 05, France

来源：

FEBS LETTERS | 1999年 / 460卷 / 02期

关键词：

DNA methylation; genome instability; DNA methyltransferase I; DNA demethylase; breast cancer;

D O I：

10.1016/S0014-5793(99)01358-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We show that in a series of eight breast cancer cell lines, a direct relationship exists between the overall DNA demethylation and the percentage of rearranged chromosomes, except for cell lines with a highly rearranged genome which can be weakly demethylated, A real time fluorescent detection method was used to quantify by reverse transcription-PCR the expression of the DNA methyltransferase 1 and of the newly discovered DNA demethylase, The overall DNA methylation status seems to result from a complex interplay between the expression of these two genes. Our results suggest that in these tumor cells, the overall DNA demethylation is implicated in one of the mechanisms at the origin of the genome instability and that besides the role of the DNA methyltransferase 1, that of the DNA demethylase may be essential in the control of DNA methylation. (C) 1999 Federation of European Biochemical Societies.

引用

页码：231 / 234

页数：4

共 23 条

[1]

ALMEIDA A, 1993, HUM GENET, V91, P538

[2]

Baylin SB, 1998, ADV CANCER RES, V72, P141

[3] DNA hypomethylation in breast cancer: An independent parameter of tumor progression? [J].