Protein folding: Thickening the broth

被引：50

作者：

Minton, AP ^{[1
]}

机构：

[1] NIH, Bethesda, MD 20892 USA

来源：

CURRENT BIOLOGY | 2000年 / 10卷 / 03期

关键词：

D O I：

10.1016/S0960-9822(00)00301-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent results support the notion that macromolecular 'crowding' enhances protein aggregation, at the expense of correct folding. The results can be rationalised in terms of kinetic competition between distinct processes, taking into account the relative influence of crowding on each process.

引用

收藏

页码：R97 / R99

页数：3

相关论文

共 15 条

[1]

Dobson Christopher M., 1999, Current Opinion in Structural Biology, V9, P92, DOI 10.1016/S0959-440X(99)80012-8

[2]

Ellis R. John, 1999, Current Opinion in Structural Biology, V9, P102, DOI 10.1016/S0959-440X(99)80013-X

[3] Molecular chaperones: Avoiding the crowd [J].

Ellis, RJ .

CURRENT BIOLOGY, 1997, 7 (09) :R531-R533

[4] PROTEIN FOLDING IN THE CELL [J].

GETHING, MJ ;

SAMBROOK, J .

NATURE, 1992, 355 (6355) :33-45

[5] A KINETIC-STUDY OF THE COMPETITION BETWEEN RENATURATION AND AGGREGATION DURING THE REFOLDING OF DENATURED REDUCED EGG-WHITE LYSOZYME [J].

GOLDBERG, ME ;

RUDOLPH, R ;

JAENICKE, R .

BIOCHEMISTRY, 1991, 30 (11) :2790-2797

[6] Molecular chaperones in cellular protein folding [J].

Hartl, FU .

NATURE, 1996, 381 (6583) :571-580

[7] Effect of a concentrated "inert" macromolecular cosolute on the stability of a globular protein with respect to denaturation by heat and by chaotropes: A statistical-thermodynamical model [J].

Minton, AP .

BIOPHYSICAL JOURNAL, 2000, 78 (01) :101-109

[8] CONFINEMENT AS A DETERMINANT OF MACROMOLECULAR STRUCTURE AND REACTIVITY .2. EFFECTS OF WEAKLY ATTRACTIVE INTERACTIONS BETWEEN CONFINED MACROSOLUTES AND CONFINING STRUCTURES [J].

MINTON, AP .

BIOPHYSICAL JOURNAL, 1995, 68 (04) :1311-1322

[9] NONSPECIFIC STABILIZATION OF STRESS-SUSCEPTIBLE PROTEINS BY STRESS-RESISTANT PROTEINS - A MODEL FOR THE BIOLOGICAL ROLE OF HEAT-SHOCK PROTEINS [J].

MINTON, KW ;

KARMIN, P ;

HAHN, GM ;

MINTON, AP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (23) :7107-7111

[10] Protein folding in the cytosol: chaperonin-dependent and -independent mechanisms [J].

Netzer, WJ ;

Hartl, FU .

TRENDS IN BIOCHEMICAL SCIENCES, 1998, 23 (02) :68-73