Cyclin-dependent kinase 8 is expressed both maternally and zygotically during zebrafish embryo development

被引：4

作者：

Brabazon, ED

Bree, RT

Carton, MW

Grealy, M

Byrnes, L ^{[1
]}

机构：

[1] Natl Univ Ireland Univ Coll Galway, Dept Biochem, Galway, Ireland

[2] Natl Univ Ireland Univ Coll Galway, Natl Ctr Biomed Engn Sci, Galway, Ireland

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2002年 / 1576卷 / 1-2期

关键词：

cyclin-dependent kinase 8; cDNA; expression; zebrafish; embryogenesis; mid-blastula transition;

D O I：

10.1016/S0167-4781(02)00302-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cyclin-dependent kinase 8 (cdk8) regulates transcription by phosphorylating RNA polymerase II and TFIIH. The mechanism of zygotic transcription activation during vertebrate embryonic development is poorly understood. Here we describe the cloning and developmental expression pattern of zebrafish cdk8 mRNA. It is highly conserved, sharing 79% DNA and 95% amino acid sequence identity with human cdk8, thereby indicating an important role for the protein. Northern blotting and whole mount in situ hybridisation revealed expression of zebrafish cdk8 maternally, following the onset of zygotic transcription at the mid-blastula transition (MBT) and throughout embryonic development. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：203 / 208

页数：6

共 28 条

[1] TFIIH is negatively regulated by cdk8-containing mediator complexes [J].