Role of mammalian Mre11 in classical and alternative nonhomologous end joining

被引：270

作者：

Xie, Anyong ^{[1
]}

Kwok, Amy

Scully, Ralph

机构：

[1] Harvard Univ, Sch Med, Dept Med, Boston, MA 02115 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2009年 / 16卷 / 08期

关键词：

STRAND-BREAK REPAIR; CLASS SWITCH RECOMBINATION; DNA-DAMAGE-RESPONSE; V(D)J RECOMBINATION; HISTONE H2AX; HOMOLOGOUS RECOMBINATION; SACCHAROMYCES-CEREVISIAE; GENOMIC INSTABILITY; IONIZING-RADIATION; LENGTH MAINTENANCE;

D O I：

10.1038/nsmb.1640

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mammalian Mre11-Rad50-Nbs1 (MRN) complex coordinates double-strand break signaling with repair by homologous recombination and is associated with the H2A.X chromatin response to double-strand breaks, but its role in nonhomologous end joining (NHEJ) is less clear. Here we show that Mre11 promotes efficient NHEJ in both wild-type and Xrcc4(-/-) mouse embryonic stem cells. Depletion of Mre11 reduces the use of microhomology during NHEJ in Xrcc4(+/+) cells and suppresses end resection in Xrcc4(-/-) cells, revealing specific roles for Mre11 in both classical and alternative NHEJ. The NHEJ function of Mre11 is independent of H2A.X. We propose a model in which both enzymatic and scaffolding functions of Mre11 cooperate to support mammalian NHEJ.

引用

页码：814 / U31

页数：6

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