Genetic characterization of antimicrobial resistance in Canadian isolates of Salmonella serovar typhimurium DT104

被引：157

作者：

Ng, LK ^{[1
]}

Mulvey, MR ^{[1
]}

Martin, I ^{[1
]}

Peters, GA ^{[1
]}

Johnson, W ^{[1
]}

机构：

[1] Hlth Canada, Bur Microbiol, Gonococcal Infect Sect, Lab Ctr Dis Control, Winnipeg, MB R3E 3R2, Canada

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1999年 / 43卷 / 12期

关键词：

D O I：

10.1128/AAC.43.12.3018

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

PCR was used to identify antibiotic resistance determinants in 31 Canadian Salmonella serovar Typhimurium DT104 isolates. Genes encoding resistance to ampicillin (pse1 or blaP1), chloramphenicol (pasppflo-like), streptomycin-spectinomycin (aadA2), sulfonamide (sulI), and tetracycline [tet(G)] Here mapped to a 13-kb region of DNA of one isolate. Two copies of sulI were identified and mapped to the 3' end of either pse1 or aadA2 integrons. The two integrons were separated by the pasppflo-like gene and the tet(G) gene. The kanamycin resistance determinant (aphA-1) was present on a 2.0-MDa plasmid (five isolates) or on the chromosome (three isolates).

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页码：3018 / 3021

页数：4

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