Calcium transport by sarcoplasmic reticulum Ca-ATPase can be investigated on a solid-supported membrane

被引:6
作者
Bartolommei, G [1 ]
Buoninsegni, FT [1 ]
Moncelli, MR [1 ]
机构
[1] Univ Florence, Dept Chem, Lab Bioelectrochem, I-50019 Sesto Fiorentino, Florence, Italy
关键词
sarcoplasmic reticulum Ca-ATPase; active transport; solid-supported membrane; capacitive current transients;
D O I
10.1016/j.bioelechem.2003.07.010
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Sarcoplasmic reticulum (SR) native vesicles incorporating Ca-ATPase are adsorbed on a solid-supported lipid membrane (SSM). Upon adsorption, the ion pumps are chemically activated by concentration jumps of ATP and the capacitive current transients generated by SR Ca-ATPase are measured under potentiostatic conditions. The Michaelis-Menten constant, Km, for ATP is evaluated by varying the concentration of ATP in the activating solution. This preliminary result shows that ion transport by SR Ca-ATPase can be suitably investigated by a technique based on concentration jumps on an SSM. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:157 / 160
页数:4
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