Fluorescent-Protein-Based Biosensors: Modulation of Energy Transfer as a Design Principle

被引：75

作者：

Campbell, Robert E. ^{[1
]}

机构：

[1] Univ Alberta, Dept Chem, Edmonton, AB T6G 2G2, Canada

来源：

ANALYTICAL CHEMISTRY | 2009年 / 81卷 / 15期

基金：

加拿大自然科学与工程研究理事会;

关键词：

CORRELATION SPECTROSCOPY; FRET; CELLS; REPORTERS; SENSOR; CA2+; NANOSENSORS; INDICATORS; OPTIMIZATION; SENSITIVITY;

D O I：

10.1021/ac802613w

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Genetically-encoded biosensors based on FRET between fluorescent proteins of different hues enable quantitative measurement of intracellular enzyme activities and small molecule concentrations. (To listen to a podcast about this feature, please go to the Analytical Chemistry website at pubs.acs.org/journal/ancham.)

引用

页码：5972 / 5979

页数：8

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