Improved regeneration efficiency from mature embryos of barley cultivars

被引：22

作者：

Akula, C ^{[1
]}

Akula, A ^{[1
]}

Henry, R ^{[1
]}

机构：

[1] So Cross Univ Lismore, CRC Mol Plant Breeding, Ctr Plant Conservat Genet, Lismore, NSW 2480, Australia

来源：

BIOLOGIA PLANTARUM | 1999年 / 42卷 / 04期

关键词：

Hordeum vulgare; tissue culture; callus induction; genotypes;

D O I：

10.1023/A:1002694410575

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A reliable protocol for plant regeneration from mature embryo derived calli of nine barley (Hordeum vulgare) cultivars has been developed, The auxins 2,4-dichlorophenoxyacetic acid, picloram and dicamba proved effective in inducing callus from mature embryos of most of the barley cultivars, The induced primary callus was loose, friable and translucent, It ultimately yielded creamy white and compact callus after 2 - 3 transfers on fresh medium of the same composition. Callus induction and regeneration capacity were highly cultivar dependent, Addition of a high concentration of picloram (4 mg. dm(-3)) promoted regeneration in 3 cultivars (Tallon, Grimmett and Sloop), In cv, Arapiles, abscisic acid and betaine were crucial in generating morphogenic callus from the mature embryos, Plants regenerated from these calli were hardy and developed roots readily when transferred to hormone free medium.

引用

页码：505 / 513

页数：9

共 26 条

[1] An efficient transformation system for the Australian rice cultivar, Jarrah [J].