The structure of recombinant human annexin VI in crystals and membrane-bound

被引：86

作者：

Benz, J ^{[1
]}

Bergner, A ^{[1
]}

Hofmann, A ^{[1
]}

Demange, P ^{[1
]}

Gottig, P ^{[1
]}

Liemann, S ^{[1
]}

Huber, R ^{[1
]}

Voges, D ^{[1
]}

机构：

[1] MAX PLANCK INST BIOCHEM,ABT STRUKTURFORSCH,D-82152 MARTINSRIED,GERMANY

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1996年 / 260卷 / 05期

关键词：

annexin; crystal structure; electron microscopy; ion channel activity;

D O I：

10.1006/jmbi.1996.0426

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The crystal structure of calcium-free recombinant human annexin VI was solved at a resolution of 3.2 Angstrom by using the annexin I model for Patterson search and refined to an X-factor of 19.0%. The molecule consists of two similar halves closely resembling annexin I connected by an a-helical segment and arranged perpendicular to each other. The calcium and membrane binding sites assigned by structural homology are therefore not located in the same plane. Analysis of the membrane-bound form of annexin VI by electron microscopy shows the two halves of the molecule coplanar with the membrane, but oriented differently to the crystal structure and suggesting a flexible arrangement. Ion channel activity has been found for annexin VI and the half molecules by electrophysiological experiments. (C) 1996 Academic Press Limited

引用

页码：638 / 643

页数：6

共 38 条

[1]

[Anonymous], ACTA CRYSTALLOGR D

[2] STRUCTURE-FUNCTION ANALYSIS OF THE ION-CHANNEL SELECTIVITY FILTER IN HUMAN ANNEXIN-V [J].

BERENDES, R ;

VOGES, D ;

DEMANGE, P ;

HUBER, R ;

BURGER, A .

SCIENCE, 1993, 262 (5132) :427-430

[3]

Brunger A. T., 1992, X PLOR VERSION 3 1 S

[4] STRUCTURAL AND ELECTROPHYSIOLOGICAL ANALYSIS OF ANNEXIN-V MUTANTS - MUTAGENESIS OF HUMAN ANNEXIN-V, AN IN-VITRO VOLTAGE-GATED CALCIUM-CHANNEL, PROVIDES INFORMATION ABOUT THE STRUCTURAL FEATURES OF THE ION PATHWAY, THE VOLTAGE SENSOR AND THE ION SELECTIVITY FILTER [J].

BURGER, A ;

VOGES, D ;

DEMANGE, P ;

PEREZ, CR ;

HUBER, R ;

BERENDES, R .

JOURNAL OF MOLECULAR BIOLOGY, 1994, 237 (04) :479-499

[5] A RAPID AND EFFICIENT PURIFICATION METHOD FOR RECOMBINANT ANNEXIN-V FOR BIOPHYSICAL STUDIES [J].

BURGER, A ;

BERENDES, R ;

VOGES, D ;

HUBER, R ;

DEMANGE, P .

FEBS LETTERS, 1993, 329 (1-2) :25-28

[6] A MODEL OF THE STRUCTURE OF HUMAN ANNEXIN-VI BOUND TO LIPID MONOLAYERS [J].

DRIESSEN, HPC ;

NEWMAN, RH ;

FREEMONT, PS ;

CRUMPTON, MJ .

FEBS LETTERS, 1992, 306 (01) :75-79

[7] ACCURATE BOND AND ANGLE PARAMETERS FOR X-RAY PROTEIN-STRUCTURE REFINEMENT [J].

ENGH, RA ;

HUBER, R .

ACTA CRYSTALLOGRAPHICA SECTION A, 1991, 47 :392-400

[8] MICROCRYSTALS OF THE ANNEXIN, P68 - PARACRYSTAL TO CRYSTAL TRANSITION AND MOLECULAR PACKING AS DETERMINED BY ELECTRON-MICROSCOPY AND IMAGE-RECONSTRUCTION [J].

GABRIEL, BL ;

TAYLOR, K ;

CREUTZ, CE ;

KRETSINGER, RH .

JOURNAL OF STRUCTURAL BIOLOGY, 1991, 107 (01) :29-37

[9] IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES [J].

HAMILL, OP ;

MARTY, A ;

NEHER, E ;

SAKMANN, B ;

SIGWORTH, FJ .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1981, 391 (02) :85-100

[10] ANNEXIN-VI IS ASSOCIATED WITH CALCIUM-SEQUESTERING ORGANELLES [J].

HAZARIKA, P ;

KAETZEL, MA ;

SHELDON, A ;

KARIN, NJ ;

FLEISCHER, S ;

NELSON, TE ;

DEDMAN, JR .

JOURNAL OF CELLULAR BIOCHEMISTRY, 1991, 46 (01) :78-85

← 1 2 3 4 →