Towards a standard battery of microsatellite markers for the analysis of the Leishmania donovani complex

被引：45

作者：

Jamjoom, MB

Ashford, RW

Bates, PA

Kemp, SJ

Noyes, HA

机构：

[1] Univ Liverpool, Sch Biol Sci, Liverpool L69 7ZD, Merseyside, England

[2] Univ Liverpool, Liverpool Sch Trop Med, Liverpool L3 5QA, Merseyside, England

来源：

ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY | 2002年 / 96卷 / 03期

关键词：

D O I：

10.1179/000349802125000790

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

The investigation of microsatellite markers has recently superseded that of isoenzymes for many population-biology applications. Microsatellites have the advantages of being dominant, neutral, highly polymorphic and easily scored by high-throughput methods. However, it is necessary to develop a new panel of markers for each group of organisms of interest. Previously, only about 5% of the markers that amplify Leishmania major microsatellite loci were also found to amplify L. donovani loci. A panel of 20 microsatellite markers that are polymorphic in L. donovani and L. infantum has now been developed, using a rapid-enrichment method that will be suitable for developing libraries of markers for other trypanosomatid species. This is the first panel of polymorphic microsatellite markers, to be isolated de novo from any species of Leishmania, that is large enough for population-biology applications.

引用

页码：265 / 270

页数：6

共 15 条

[1] ESTIMATION OF POPULATION AT RISK OF INFECTION AND NUMBER OF CASES OF LEISHMANIASIS [J].

ASHFORD, RW ;

DESJEUX, P ;

DERAADT, P .

PARASITOLOGY TODAY, 1992, 8 (03) :104-105

[2] Microsatellites: consensus and controversy [J].

Chambers, GK ;

MacAvoy, ES .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 2000, 126 (04) :455-476

[3] Leishmaniasis - Public health aspects and control [J].

Desjeux, P .

CLINICS IN DERMATOLOGY, 1996, 14 (05) :417-423

[4] Isolation of microsatellite loci from a social lizard, Egernia stokesii, using a modified enrichment procedure [J].

Gardner, MG ;

Cooper, SJB ;

Bull, CM ;

Grant, WN .

JOURNAL OF HEREDITY, 1999, 90 (02) :301-304

[5]

JAMJOOM MB, 2002, IN PRESS MOL ECOLOGY

[6]

Lainson R., 1987, The Leishmaniases in Biology and Medicine, P1

[7] The strange case of Leishmania chagasi [J].

Maurício, IL ;

Stothard, JR ;

Miles, MA .

PARASITOLOGY TODAY, 2000, 16 (05) :188-189

[8] Sudan: the possible original focus of visceral leishmaniasis [J].

Pratlong, F ;

Dereure, J ;

Bucheton, B ;

El-Safi, S ;

Dessein, A ;

Lanotte, G ;

Dedet, JP .

PARASITOLOGY, 2001, 122 :599-605

[9]

Rioux JA, 1986, LEISHMANIA TAXONOMIE, P485

[10] STRUCTURAL ORGANIZATION OF MICROSATELLITE FAMILIES IN THE LEISHMANIA GENOME AND POLYMORPHISMS AT 2 (CA)(N) LOCI [J].

ROSSI, V ;

WINCKER, P ;

RAVEL, C ;

BLAINEAU, C ;

PAGES, M ;

BASTIEN, P .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1994, 65 (02) :271-282

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