Production of constitutive, thermostable, hyperactive exo-pectinase from Bacillus GK-8

被引:23
作者
Dosanjh, NS
Hoondal, GS
机构
关键词
D O I
10.1007/BF00129350
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A new alkalophilic Bacillus GK-8 overproduced three thermostable extracellular pectinases. The temperature optima was 60 degrees C for all the three enzymes which retained full activity for 2 h. They had pH optima 5.4, 7.0 and 10.4 and were designated as PT, PII and PIII respectively. The half life at 80 degrees C of PI, PII and PIII was 18 min., 12 min. and 12 min., respectively. The enzyme activity was stimulated by Mg2+, Ca2+, Zn2+, Co2+ and Mn2+ ions.
引用
收藏
页码:1435 / 1438
页数:4
相关论文
共 17 条
[1]   CONSTITUTIVE EXO-PECTINASE PRODUCED BY ASPERGILLUS SP CH-Y-1043 ON DIFFERENT CARBON SOURCE [J].
AGUILAR, G ;
HUITRON, C .
BIOTECHNOLOGY LETTERS, 1990, 12 (09) :655-660
[2]   DETECTION OF PECTIC ENZYMES IN PECTIN-ACRYLAMIDE GELS [J].
CRUICKSHANK, RH ;
WADE, GC .
ANALYTICAL BIOCHEMISTRY, 1980, 107 (01) :177-181
[3]   ENHANCED POLYGALACTURONASE PRODUCTION BY ASPERGILLUS-NIGER NRRL-364 GROWN ON SUPPLEMENTED CITRUS PECTIN [J].
GALIOTOUPANAYOTOU, M ;
RODIS, P ;
KAPANTAI, M .
LETTERS IN APPLIED MICROBIOLOGY, 1993, 17 (04) :145-148
[4]   PRODUCTION OF ALKALINE ENZYMES BY ALKALOPHILIC MICROORGANISMS .3. ALKALINE PECTINASE OF BACILLUS NO P-4-N [J].
HORIKOSHI, K .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1972, 36 (02) :285-+
[5]   PURIFICATION AND PROPERTIES OF POLYGALACTURONIC ACID TRANS-ELIMINASE FROM BACILLUS-STEAROTHERMOPHILUS [J].
KARBASSI, A ;
VAUGHN, RH .
CANADIAN JOURNAL OF MICROBIOLOGY, 1980, 26 (03) :377-384
[6]   PRODUCTION AND PROPERTIES OF POLYGALACTURONATE LYASE BY AN ALKALOPHILIC MICROORGANISM BACILLUS-SP-RK9 [J].
KELLY, CT ;
FOGARTY, WM .
CANADIAN JOURNAL OF MICROBIOLOGY, 1978, 24 (10) :1164-1172
[7]  
Kelly CT., 1990, MICROBIAL ENZYMES BI, V2, P275
[8]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[9]  
MAGRO P, 1994, FEMS MICROBIOL LETT, V117, P1, DOI 10.1111/j.1574-6968.1994.tb06733.x
[10]   USE OF DINITROSALICYLIC ACID REAGENT FOR DETERMINATION OF REDUCING SUGAR [J].
MILLER, GL .
ANALYTICAL CHEMISTRY, 1959, 31 (03) :426-428