A microfluidic electrochemical device for high sensitivity biosensing: Detection of nanomolar hydrogen peroxide

被引：63

作者：

Chikkaveeraiah, Bhaskara V. ^{[1
]}

Liu, Hongyun ^{[1
,3
]}

Mani, Vigneshwaran ^{[1
]}

Papadimitrakopoulos, Fotios ^{[1
]}

Rusling, James F. ^{[1
,2
,4
]}

机构：

[1] Univ Connecticut, Inst Mat Sci, Dept Chem, Storrs, CT 06269 USA

[2] Univ Connecticut, Ctr Hlth, Farmington, CT 06032 USA

[3] Beijing Normal Univ, Dept Chem, Beijing 100875, Peoples R China

[4] Natl Univ Ireland, Sch Chem, Galway, Ireland

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2009年 / 11卷 / 04期

基金：

爱尔兰科学基金会;

关键词：

Microfluidics; Biosensor; H2O2; Electrocatalysis; Direct electron transfer; MICROCHIP; DIFFUSION; IMMUNOASSAY; VOLTAMMETRY; FABRICATION; SEPARATION; INJECTION; SYSTEMS; SURFACE;

D O I：

10.1016/j.elecom.2009.02.002

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

We report herein a simple device for rapid biosensing consisting of a single microfluidic channel made from poly(dimethylsiloxane) (PDMS) coupled to an injector, and incorporating a biocatalytic sensing electrode, reference and counter electrodes. The sensing electrode was a gold wire coated with 5 nm glutathione-decorated gold nanoparticles (AuNPs). Sensitive detection of H2O2 based on direct bioelectrocatalysis by horseradish peroxidase (HRP) was used for evaluation. HRP was covalently linked the glutathione-AuNPs. This electrode presented quasi-reversible cyclic voltammetry peaks at -0.01 V vs. Ag/AgCl at pH 6.5 for the HRP heme Fe-III/Fe-II couple. Direct electrochemical activity of HRP was used to detect H2O2 at high sensitivity with a detection limit of 5 nM in an unmediated system. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：819 / 822

页数：4

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