Lack of a chromosomal copy of the circular rDNA plasmid of Entamoeba histolytica

被引：22

作者：

Bagchi, A

Bhattacharya, A

Bhattacharya, S ^{[1
]}

机构：

[1] Jawaharlal Nehru Univ, Sch Environm Sci, New Delhi 110067, India

[2] Jawaharlal Nehru Univ, Sch Life Sci, New Delhi 110067, India

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1999年 / 29卷 / 11期

关键词：

anomalous migration; circular DNA; Entamoeba histolytica; exonuclease Bal31; extrachromosomal rDNA; genome mapping; molecular karyotype; pulsed field gel electrophoresis;

D O I：

10.1016/S0020-7519(99)00125-3

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A number of small circular DNAs constitute a part of the genome of Entomoeba histolytica. Among them, the 24.5 kb circular DNA encoding rRNA (EhR1) is the most abundant. Pulsed field gel electrophoresis was used to determine if a chromosomal copy of EhR1 exists and what fraction of the total genome is circular. The results show that the chromosomes of E. histolytica are linear, and that no copy of EhR1 could be detected in any of the linear chromosomes. (C) 1999 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.

引用

页码：1775 / 1783

页数：9

共 29 条

[1] Identification of novel genes from Entamoeba histolytica by expressed sequence tag analysis [J].